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Journal of Clinical Microbiology, January 2000, p. 61-67, Vol. 38, No. 1
0095-1137/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

New Monoclonal Antibody Specific for Candida albicans Germ Tube

Agnes Marot-Leblond,1,* Linda Grimaud,1 Sandrine Nail,1 Sandrine Bouterige,1 Veronique Apaire-Marchais,1 Derek J. Sullivan,2 and Raymond Robert1

Groupe d'Etude des Interactions Hôte-Parasite, UFR des Sciences Pharmaceutiques et d'Ingénierie de la Santé, 49100 Angers, France,1 and Department of Oral Surgery, Oral Medicine and Oral Pathology, School of Dental Science, Trinity College, University of Dublin, Dublin 2, Republic of Ireland2

Received 21 May 1999/Returned for modification 27 July 1999/Accepted 29 September 1999

Hydrophobic components of the germ tube of the dimorphic pathogenic fungus Candida albicans were used as immunogens to prepare monoclonal antibodies (MAbs). Among the resulting MAbs, one (MAb 16B1-F10) was shown by indirect immunofluorescence to be specific to the surface of the mycelium phase of the C. albicans and C. stellatoidea species. No labeling of any other genera and Candida species tested was observed, including C. dubliniensis, a newly described species which has many phenotypic similarities to C. albicans. This phase-specific epitope resides on a protein moiety. The molecular mass of the antigen released by Zymolyase digestion was determined by gel filtration and ranges from 25 to 166 kDa. The antigen was also shown to be highly hydrophobic. This anti-C. albicans cell wall surface-specific MAb may be a good candidate for use in tests for the rapid differentiation of the two closely related species C. albicans and C. dubliniensis.

* Corresponding author. Mailing address: Laboratoire de Mycologie, Faculté de Pharmacie, 16 boulevard Daviers, 49100 Angers, France. Phone: (33) 02 41 22 66 61. Fax: (33) 02 41 48 67 33. E-mail: agnes.marot{at}univ-angers.fr.

Journal of Clinical Microbiology, January 2000, p. 61-67, Vol. 38, No. 1
0095-1137/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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