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Journal of Clinical Microbiology, January 2000, p. 7-12, Vol. 38, No. 1
0095-1137/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Characterization of Escherichia coli
Strains from Cases of Childhood Diarrhea in Provincial
Southwestern Nigeria
Iruka N.
Okeke,1,2
Adebayo
Lamikanra,2
Hartmut
Steinrück,3 and
James B.
Kaper1,*
Department of Microbiology and Immunology and
Center for Vaccine Development, University of Maryland School of
Medicine, Baltimore, Maryland 212011;
Department of Pharmaceutics, Obafemi Awolowo University,
Ile-Ife, Nigeria2; and Robert
Koch-Institut, 13353 Berlin, Germany3
Received 11 May 1999/Returned for modification 26 July
1999/Accepted 24 September 1999
In a study carried out in small-town and rural primary health care
centers in southwestern Nigeria, 330 Escherichia coli
strains isolated from 187 children with diarrhea and 144 apparently
healthy controls were examined for virulence traits. Based on the
results of colony blot hybridization, strains were categorized as
enteropathogenic E. coli (1.8%), enterotoxigenic E. coli (2.4%), enteroinvasive E. coli (1.2%),
enterohemorrhagic E. coli (0.6%), enteroaggregative E. coli (10.3%), diffusely adherent E. coli
(7.9%), cell-detaching E. coli (6.9%), and cytolethal
distending toxin-producing E. coli (0.9%). E. coli strains that hybridized with a Shiga toxin gene probe but
lacked other characteristics usually present in enterohemorrhagic E. coli constituted 8.4% of the isolates. Ninety-seven
E. coli isolates adhered to HEp-2 cells in an aggregative
fashion but did not hybridize with any of the probes employed in the
study. Overall the pathotypes, apart from cytolethal distending
toxin-producing E. coli, were recovered both from children
with diarrhea and from children without diarrhea, though to a lower
extent from the healthy children. All diarrheagenic E. coli
strains were associated with diarrhea (P < 0.02).
Heat-stable-enterotoxin-producing enterotoxigenic E. coli
showed significant association with diarrhea (P < 0.02), as did strains that demonstrated aggregative adherence to HEp-2 cells (P < 0.04), but not those that hybridized with
the CVD432 enteroaggregative probe.
*
Corresponding author. Mailing address: Center for
Vaccine Development, University of Maryland School of Medicine, 685 West Baltimore St., Baltimore, MD 21201. Phone: (410) 706-5328. Fax: (410) 706-0182. E-mail: jkaper{at}umaryland.edu.
Journal of Clinical Microbiology, January 2000, p. 7-12, Vol. 38, No. 1
0095-1137/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
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