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Journal of Clinical Microbiology, January 2000, p. 99-104, Vol. 38, No. 1
0095-1137/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Performance of Indirect Immunoglobulin M (IgM) Serology Tests and IgM Capture Assays for Laboratory Diagnosis of Measles

Samuel Ratnam,1,dagger ,* Graham Tipples,2,dagger Carol Head,1 Micheline Fauvel,3,dagger Margaret Fearon,4,dagger and Brian J. Ward5,dagger

Newfoundland Public Health Laboratory, St. John's, Newfoundland,1 Viral Exanthema Laboratory, Laboratory Centre for Disease Control, Winnipeg, Manitoba,2 Laboratoire de Sante Publique du Quebec, Ste-Anne-de-Bellevue, Quebec,3 Central Public Health Laboratory, Ontario Ministry of Health, Laboratory Services Branch, Toronto, Ontario,4 and McGill Centre for the Study of Host Resistance, Montreal General Hospital, Montreal, Quebec,5 Canada

Received 4 May 1999/Returned for modification 25 August 1999/Accepted 8 October 1999

As progress is made toward elimination of measles, the laboratory confirmation of measles becomes increasingly important. However, both false-positive and false-negative results can occur with the routinely used indirect measles immunoglobulin M (IgM) serology tests. The measles IgM capture assay is considered to be more specific, and therefore, its use is indicated for confirmatory testing, but its relative performance has not been fully assessed. Four commercial indirect measles IgM serology test kits (the Behring, Clark, Gull, and PanBio assays) and a commercial IgM capture assay (the Light Diagnostics assay) were evaluated for their abilities to detect measles virus-specific IgM antibody with a total of 308 serum samples from patients involved in a measles outbreak and with confirmed cases of measles and 454 samples from subjects without measles. The Centers for Disease Control and Prevention (CDC) IgM capture assay was also used in a part of the evaluation. Among the indirect assays, the overall sensitivities ranged from 82.8% (Clark assay) to 88.6% (Behring assay) and specificity ranged from 86.6% (PanBio assay) to 99.6% (Gull assay). These rates were 92.2 and 86.6%, respectively, for the Light Diagnostics capture assay and 87.0 and 94.8%, respectively, for the CDC capture assay. While the Light Diagnostics capture assay had the best detection rate (80%) with the acute-phase samples compared with those for the rest of the tests (CDC capture assay, 77%; Behring assay, 70%; Gull assay, 69%; PanBio assay, 58%; and Clark assay, 57%), all tests showed a significantly improved sensitivity in the range of 92% (Clark and PanBio assays) to 97% (Light Diagnostics and CDC capture assays) with the convalescent-phase samples, as expected. The best seropositivity rates (in the range of 92 to 100%) were observed with samples collected 6 to 14 days after the onset of symptoms. The Gull assay showed the highest positive predictive value (99.6%), followed by the Behring assay (97.8%) and the CDC capture assay (96.1%). Overall, the Gull and Behring assays were found to be as good as or better than the capture assays. In conclusion, laboratory diagnosis of measles based on IgM serology varies depending on the timing of specimen collection and the test used, and the case for the use of the IgM capture assay as the confirmatory test appears to be uncertain.

* Corresponding author. Mailing address: Public Health Laboratory, Leonard A. Miller Centre, St. John's, NF, Canada A1B 3T2. Phone: (709) 737-6565. Fax: (709) 737-7070 or (709) 737-6611. E-mail: nphlab{at}newcomm.net.

dagger Member of the Laboratory Subcommittee, Working Group on Measles Elimination in Canada.

Journal of Clinical Microbiology, January 2000, p. 99-104, Vol. 38, No. 1
0095-1137/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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