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Journal of Clinical Microbiology, October 2000, p. 3572-3576, Vol. 38, No. 10
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Spoligotyping and Polymorphic GC-Rich Repetitive Sequence Fingerprinting of Mycobacterium tuberculosis Strains Having Few Copies of IS6110

Z. H. Yang,1,2 K. Ijaz,3 J. H. Bates,1,2,3,4 K. D. Eisenach,1,4,5 and M. D. Cave1,6,*

Regional Tuberculosis Genotyping Laboratory1 and Departments of Medicine,2 Microbiology/Immunology,4 Pathology,5 and Anatomy,6 University of Arkansas for Medical Sciences, and Central Arkansas Veterans Healthcare System, Arkansas Department of Health,3 Little Rock, Arkansas

Received 22 March 2000/Returned for modification 10 July 2000/Accepted 18 July 2000

Several genetic loci have been utilized to genotype isolates of Mycobacterium tuberculosis. A shortcoming of the most commonly used method, IS6110 fingerprinting, is that it does not adequately discriminate between isolates having few copies of IS6110. This study was undertaken to compare pTBN12 fingerprinting of polymorphic GC-rich repetitive sequence genes and spoligotyping of the direct repeat locus as secondary typing procedures for M. tuberculosis isolates having fewer than six copies of IS6110. A total of 88 isolates (100% of the isolates with fewer than six copies of IS6110 isolated in Arkansas during 1996 and 1997) were included in this study. Among the 88 isolates, 34 different IS6110 patterns were observed, 10 of which were shared by more than 1 isolate, involving a total of 64 isolates. The 64 isolates were subdivided into 13 clusters (containing 37 isolates) and 27 unique isolates based on a combination of IS6110 and pTBN12 fingerprinting and into 11 clusters (containing 51 isolates) and 13 unique isolates based on a combination of IS6110 fingerprinting and spoligotyping. Identical spoligotypes were found among isolates having different IS6110 patterns, as well as among isolates showing different pTBN12 patterns. In contrast, all isolates that had different IS6110 patterns were found to be unique by pTBN12 typing. The clustering rate was 73, 58, and 42%, respectively, for IS6110 fingerprinting alone, IS6110 fingerprinting and spoligotyping combined, and IS6110 and pTBN12 combined fingerprinting. The data indicate that the pTBN12 method has greater discriminating power among low-copy-number isolates than does spoligotyping.

* Corresponding author. Mailing address: Central Arkansas Veterans Healthcare System, Medical Research Service, LR/151, 4300 West 7th St., Little Rock, AR 72205. Phone: (501) 257-4829. Fax: (501) 664-6748. E-mail: cavedonald{at}exchange.uams.edu.

Journal of Clinical Microbiology, October 2000, p. 3572-3576, Vol. 38, No. 10
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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