Previous Article | Next Article ![]()
Journal of Clinical Microbiology, October 2000, p. 3631-3635, Vol. 38, No. 10
Danish Veterinary Laboratory, DK-8200 Århus
N, Denmark
Received 28 February 2000/Returned for modification 26 June
2000/Accepted 27 July 2000
During the years 1994 to 1998, 10 strains of Salmonella
enterica serovar Enteritidis phage type 11 (PT11) and 6 PT9a
strains were isolated from Danish hedgehogs, together with 7 strains
that did not yield phage susceptibility patterns conforming with any known phage type (routine dilution no conformity [RDNC]). From 1995 to 1998, five Danish patients were reported infected with serovar
Enteritidis PT11 and two with PT9a. All serovar Enteritidis PT11, PT9a,
and RDNC isolates from hedgehogs and humans were analyzed by
pulsed-field gel electrophoresis (PFGE), plasmid profiling, and
restriction fragment length polymorphism (RFLP) of plasmids. By use of
S1 nuclease and HindIII, the PT11 and PT9a isolates had
identical plasmid profiles and RFLP patterns, which differed from the
RDNC profiles. The PFGE profiles were identical for all serovar
Enteritidis PT11 and PT9a strains from hedgehogs, four of five human
strains of serovar Enteritidis PT11, and two human strains of serovar
Enteritidis PT9a, irrespective of restriction enzyme, whereas the last
human strain deviated slightly when NotI was used but not
when XbaI or SpeI was used. The results
indicate that serovar Enteritidis PT9a and PT11 are closely related and that PT11 and PT9a from Danish hedgehogs and humans belong to the same
clonal lineage.
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Comparison of Danish Isolates of Salmonella enterica
Serovar Enteritidis PT9a and PT11 from Hedgehogs (Erinaceus
europaeus) and Humans by Plasmid Profiling and Pulsed-Field
Gel Electrophoresis
*
Corresponding author. Mailing address: Danish
Veterinary Laboratory, Hangøvej 2, DK-8200 Aarhus N, Denmark. Phone:
45 89 37 24 93. Fax: 45 89 37 24 48. E-mail: kpe{at}svs.dk.
Copyright © 2009 by the American Society for Microbiology. For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»