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Journal of Clinical Microbiology, October 2000, p. 3718-3721, Vol. 38, No. 10
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Immunodiagnosis of Echinococcus Infections: Confirmatory Testing and Species Differentiation by a New Commercial Western Blot

Martine Liance,1,* Veronique Janin,2 Solange Bresson-Hadni,2 Dominique-Angele Vuitton,2 Rene Houin,1 and Renaud Piarroux2

Laboratoire de Parasitologie-Mycologie, Hôpital Henri Mondor AP-HP, et Université Paris XII, 94010 Créteil,1 and Santé et Environnement Rural, Université de Franche-Comté, 25030, Besançon,2 France

Received 2 May 2000/Returned for modification 28 June 2000/Accepted 27 July 2000

The Echinococcus Western Blot IgG (LDBIO Diagnostics, Lyon, France), using a whole larval antigen from Echinococcus multilocularis, was evaluated for serodiagnosis and differentiation between two human parasitic infections of worldwide importance: cystic echinococcosis, due to Echinococcus granulosus, and alveolar echinococcosis, due to E. multilocularis. Fifty and 61 serum samples from patients with cystic and alveolar echinococcosis, respectively, were used for assessing diagnostic sensitivity. The sensitivity of the assay was compared with those of screening tests used for these applications. Sera used for assessing cross-reactivities were from 154 patients with other diseases, either parasitic or not. The assay allowed the detection of serum immunoglobulin G antibodies in 97% of Echinococcus-infected patients. It had a higher sensitivity than screening assays for the detection for each echinococcosis. The assay allowed us to correctly distinguish between E. granulosus- and E. multilocularis-infected patients in 76% of cases. It did not allow us to distinguish active from inactive forms of both echinococcoses. The occurrence of cross-reactivities with neurocysticercosis indicates the necessity for retesting sera with species-specific antigens, for rare patients with neurologic disorders. This study shows the usefulness of the commercially available Echinococcus Western Blot IgG for the serological confirmation of human echinococcosis.

* Corresponding author. Mailing address: Laboratoire de Parasitologie-Mycologie, Hôpital Henri Mondor AP-HP, 51, Avenue du Maréchal de Lattre de Tassigny, 94000 Créteil, France. Phone: 33 1 49 81 36 11. Fax: 33 1 49 81 36 01. E-mail: liance{at}univ-paris12.fr.

Journal of Clinical Microbiology, October 2000, p. 3718-3721, Vol. 38, No. 10
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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