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Journal of Clinical Microbiology, October 2000, p. 3746-3749, Vol. 38, No. 10
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Characterization of Staphylococcus aureus Coagulase Type VII Isolates from Staphylococcal Food Poisoning Outbreaks (1980-1995) in Tokyo, Japan, by Pulsed-Field Gel Electrophoresis

Akira Shimizu,1,* Manabu Fujita,1 Hideo Igarashi,2 Michihiro Takagi,1 Naoko Nagase,1 Asako Sasaki,1 and Junichi Kawano1

Department of Microbiology and Immunology, Faculty of Agriculture, Kobe University, Nada-ku, Kobe-shi, Hyogo 657-0013,1 and Department of Food and Nutrition, Kokusai Gakuin Saitama Junior College, Ohmiya-shi, Saitama 330-8548,2 Japan

Received 18 April 2000/Returned for modification 16 June 2000/Accepted 1 August 2000

Staphylococcus aureus coagulase type VII strains have been the strains most frequently isolated from staphylococcal food poisoning outbreaks in Tokyo, Japan. We applied pulsed-field gel electrophoresis (PFGE) of chromosomal DNA digested with SmaI to characterize 129 coagulase type VII strains. These were isolated from 129 cases occurring in outbreaks in 35 districts during a 16-year period (1980-1995). The 129 outbreak strains were classified into three types, designated A (n = 115), B (n = 10), and C (n = 4). Types A and C were further divided into 33 (A1 to A33) and 4 (C1 to C4) subtypes, respectively. Strains of the same subtypes were isolated from food poisoning cases in the same districts at time intervals of 1 or 2 to 5 years. PFGE typing appears to be a useful method for subdividing strains of S. aureus coagulase type VII. A combination of coagulase typing and PFGE typing would provide more detailed information than the former method alone in epidemiologic investigations of staphylococcal food poisoning.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, Faculty of Agriculture, Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe-shi, Hyogo 657-0013, Japan. Phone and fax: 81-78-803-5816. E-mail: shimizua{at}kobe-u.ac.jp.

Journal of Clinical Microbiology, October 2000, p. 3746-3749, Vol. 38, No. 10
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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