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Journal of Clinical Microbiology, October 2000, p. 3750-3754, Vol. 38, No. 10
Laboratory of Environmental Molecular
Biology, Graduate School of Environmental Earth Science, Hokkaido
University, Sapporo,1 and Department of
Preventive Dentistry, Health Sciences University of Hokkaido,
Ishikari-Tobetsu,2 Hokkaido, Japan
Received 31 May 2000/Returned for modification 27 June
2000/Accepted 25 July 2000
Previously, Rhodotorula glutinis was reported to
produce a large amount of exocellular mannan, having a
repeating unit of
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Utilization of Exocellular Mannan from Rhodotorula
glutinis as an Immunoreactive Antigen in Diagnosis of
Leptospirosis
3)-D-Manp-(14)-D-Manp-(1.
Recently, we found that antigenic polysaccharides of
Leptospira biflexa serovar patoc strain Patoc I
have the same repeating unit and cross-react with antisera raised against extended strains of other leptospires (K. Matsuo, E. Isogai, and Y. Araki, Carbohydr. Res., in press). This structural identity and
the difficulty of producing and isolating antigens led us to confirm
the usefulness of Rhodotorula mannan as an immunoreactive antigen in a serological diagnosis of leptospirosis. In the present investigation, we confirmed the structural identity of an exocellular mannan isolated from R. glutinis AHU 3479 and tried to use
it as an immunoreactive antigen in a serological diagnosis of
leptospirosis. From its chemical analysis and 1H- and
13C-labeled nuclear magnetic resonance spectrometry, the
Rhodotorula mannan was confirmed to consist of the same
disaccharide units. Furthermore, such a preparation was shown to
immunoreact to various sera from patients suffering with leptospirosis
as well as to most rabbit antiserum preparations obtained from
immunization with various strains of pathogenic leptospires. Therefore,
the Rhodotorula mannan preparation is useful as an
immunoreactive antigen in the serological diagnosis for leptospirosis.
*
Corresponding author. Mailing address: Laboratory of
Environmental Molecular Biology, Division of Bioscience, Graduate
School of Environmental Earth Science, Hokkaido University, Sapporo, Hokkaido, Japan 060-0810. Phone: 81-11-706-2231. Fax: 81-11-706-4867. E-mail: araki{at}ees.hokudai.ac.jp.
Journal of Clinical Microbiology, October 2000, p. 3750-3754, Vol. 38, No. 10
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
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