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Journal of Clinical Microbiology, October 2000, p. 3800-3810, Vol. 38, No. 10
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Evaluation of Phenotypic and Genotypic Methods for Subtyping Campylobacter jejuni Isolates from Humans, Poultry, and Cattle

Eva Møller Nielsen,1,* Jørgen Engberg,2 Vivian Fussing,2 Lise Petersen,1 Carl-Henrik Brogren,3 and Stephen L. W. On1

Danish Veterinary Laboratory, 1790 Copenhagen,1 Statens Serum Institut, 2300 Copenhagen,2 and Institute of Food Safety and Toxicology, 2860 Søborg,3 Denmark

Received 2 March 2000/Returned for modification 11 June 2000/Accepted 28 July 2000

Six methods for subtyping of Campylobacter jejuni were compared and evaluated with a collection of 90 isolates from poultry, cattle, and sporadic human clinical cases as well as from a waterborne outbreak. The applied methods were Penner heat-stable serotyping; automated ribotyping (RiboPrinting); random amplified polymorphic DNA typing (RAPD); pulsed-field gel electrophoresis (PFGE); restriction fragment length polymorphisms of the flagellin gene, flaA (fla-RFLP); and denaturing gradient gel electrophoresis of flaA (fla-DGGE). The methods were evaluated and compared on the basis of their abilities to identify isolates from one outbreak and discriminate between unrelated isolates and the agreement between methods in identifying clonal lines. All methods identified the outbreak strain. For a collection of 80 supposedly unrelated isolates, RAPD and PFGE were the most discriminatory methods, followed by fla-RFLP and RiboPrinting. fla-DGGE and serotyping were the least discriminative. All isolates included in this study were found to be typeable by each of the methods. Thirteen groups of potentially related isolates could be identified using a criterion that at least four of the methods agreed on clustering of isolates. None of the subtypes could be related to only one source; rather, these groups represented isolates from different sources. Furthermore, in two cases isolates from cattle and human patients were found to be identical according to all six methods.

* Corresponding author. Mailing address: Department of Microbiology, Danish Veterinary Laboratory, 27 Bülowsvej, DK-1790 Copenhagen, Denmark. Phone: 45 3530 0100. Fax: 45 3530 0120. E-mail: emn{at}svs.dk.

Journal of Clinical Microbiology, October 2000, p. 3800-3810, Vol. 38, No. 10
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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