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Journal of Clinical Microbiology, October 2000, p. 3800-3810, Vol. 38, No. 10
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Evaluation of Phenotypic and Genotypic Methods for
Subtyping Campylobacter jejuni Isolates from Humans,
Poultry, and Cattle
Eva Møller
Nielsen,1,*
Jørgen
Engberg,2
Vivian
Fussing,2
Lise
Petersen,1
Carl-Henrik
Brogren,3 and
Stephen
L. W.
On1
Danish Veterinary Laboratory, 1790 Copenhagen,1 Statens Serum Institut,
2300 Copenhagen,2 and Institute of
Food Safety and Toxicology, 2860 Søborg,3
Denmark
Received 2 March 2000/Returned for modification 11 June
2000/Accepted 28 July 2000
Six methods for subtyping of Campylobacter jejuni were
compared and evaluated with a collection of 90 isolates from poultry, cattle, and sporadic human clinical cases as well as from a waterborne outbreak. The applied methods were Penner heat-stable serotyping; automated ribotyping (RiboPrinting); random amplified polymorphic DNA
typing (RAPD); pulsed-field gel electrophoresis (PFGE); restriction fragment length polymorphisms of the flagellin gene, flaA
(fla-RFLP); and denaturing gradient gel electrophoresis of
flaA (fla-DGGE). The methods were evaluated and
compared on the basis of their abilities to identify isolates from one
outbreak and discriminate between unrelated isolates and the agreement
between methods in identifying clonal lines. All methods identified the
outbreak strain. For a collection of 80 supposedly unrelated isolates, RAPD and PFGE were the most discriminatory methods, followed by fla-RFLP and RiboPrinting. fla-DGGE and
serotyping were the least discriminative. All isolates included in this
study were found to be typeable by each of the methods. Thirteen groups
of potentially related isolates could be identified using a criterion
that at least four of the methods agreed on clustering of isolates.
None of the subtypes could be related to only one source; rather, these groups represented isolates from different sources. Furthermore, in two
cases isolates from cattle and human patients were found to be
identical according to all six methods.
*
Corresponding author. Mailing address: Department of
Microbiology, Danish Veterinary Laboratory, 27 Bülowsvej, DK-1790
Copenhagen, Denmark. Phone: 45 3530 0100. Fax: 45 3530 0120. E-mail:
emn{at}svs.dk.
Journal of Clinical Microbiology, October 2000, p. 3800-3810, Vol. 38, No. 10
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
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