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Journal of Clinical Microbiology, October 2000, p. 3830-3833, Vol. 38, No. 10
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Comparison between Plasma and Whole Blood Specimens for Detection of Aspergillus DNA by PCR

Juergen Loeffler,1,* Holger Hebart,1 Ulrike Brauchle,1 Ulrike Schumacher,2 and Hermann Einsele1

Medizinische Klinik, Abteilung II,1 and Hygieneinstitut,2 Eberhard-Karls-Universität Tübingen, 72076 Tübingen, Germany

Ninety-six plasma and whole blood specimens from nine selected patients were analyzed for the presence of Aspergillus DNA. Nineteen specimens from three patients with proven aspergillosis were PCR positive in both materials, whereas an additional 22 were PCR positive in whole blood only. All 36 samples from six patients without signs of aspergillosis were negative in both assays. We conclude that although plasma and whole blood spiked with Aspergillus conidia showed an identical lower detection limit (10 CFU), the sensitivity of plasma PCR was lower than that of PCR performed on whole blood samples.

* Corresponding author. Mailing address: Medizinische Klinik, Abt. II, Labor Prof. Dr. med. H. Einsele, Otfried-Mueller-Str. 10, 72076 Tuebingen, Germany. Phone: 49 7071 2987355. Fax: 49 7071 293179. E-mail: juergen.loeffler{at}med.uni-tuebingen.de.

Journal of Clinical Microbiology, October 2000, p. 3830-3833, Vol. 38, No. 10
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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