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Journal of Clinical Microbiology, October 2000, p. 3837-3839, Vol. 38, No. 10
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Distribution of a Specific 500-Base-Pair Fragment in Mycobacterium bovis Isolates from Sardinian Cattle

Leonardo A. Sechi,1,* Ilaria Duprè,1 Guido Leori,2 Giovanni Fadda,3 and Stefania Zanetti1

Dipartimento di Scienze Biomediche, Sezione di Microbiologia Sperimentale e Clinica, Università degli studi di Sassari,1 and Istituto Zooprofilattico della Sardegna,2 07100 Sassari, and Istituto di Microbiologia, Facoltà di Medicina e Chirurgia "Agostino Gemelli," Università Cattolica del Sacro Cuore, 00168 Rome,3 Italy

Received 2 September 1999/Returned for modification 16 November 1999/Accepted 11 July 2000

Amplification of a specific, 500-bp fragment from Mycobacterium bovis isolates and use of the fragment to differentiate between Mycobacterium tuberculosis and M. bovis was previously reported (J. G. Rodriguez, G. A. Meja, P. Del Portillo, M. E. Patarroyo, and L. A. Murillo, Microbiology 141:2131-2138, 1995). In the present study, 30 M. bovis isolates from Sardinian cattle were examined for the presence of this 500-bp fragment; 4 of the 30 isolates lacked the fragment. This result indicates that identification of M. bovis strains by amplification of the 500-bp sequence may lead to false-negative results.

* Corresponding author. Mailing address: Dipartimento di Scienze Biomediche, Sezione di Microbiologia Sperimentale e Clinica, Università degli studi di Sassari, Viale S. Pietro 43/B, 07100 Sassari, Italy. Phone: 79 228303. Fax: 79 212345. E-mail: sechila{at}ssmain.uniss.it.

Journal of Clinical Microbiology, October 2000, p. 3837-3839, Vol. 38, No. 10
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.




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