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Journal of Clinical Microbiology, October 2000, p. 3860-3863, Vol. 38, No. 10
0095-1137/00/$04.00+0
Comparison of Six Commercial DNA Extraction Kits for Recovery of
Cytomegalovirus DNA from Spiked Human Specimens
Gary A.
Fahle* and
Steven H.
Fischer
Microbiology Service, Clinical Pathology
Department, Warren G. Magnuson Clinical Center, National Institutes
of Health, Bethesda, Maryland 20892-1508
Received 17 March 2000/Returned for modification 10 May
2000/Accepted 17 July 2000
We evaluated six commercially available DNA extraction kits for
their ability to recover DNA from various dilutions of cytomegalovirus (CMV) added to four different specimens: bronchoalveolar lavage, cerebral spinal fluid, plasma, and whole blood. The kits evaluated included the Puregene DNA isolation kit (PG), Generation Capture Column
kit, MasterPure DNA purification kit, IsoQuick nucleic acid extraction
kit, QIAamp blood kit, and NucliSens isolation kit (NS). All six kits
evaluated effectively removed PCR inhibitors from each of the four
specimen types and produced consistently positive results down to a
spiked concentration of 200 PFU of whole CMV per ml. However, the NS
and PG resulted in the most consistently positive results at the lowest
concentrations of spiked CMV (4 and 0.4 PFU/ml) and, in this
evaluation, offered the most sensitive methods for extracting CMV DNA
from the four different spiked specimens. Processing time and cost were
also evaluated.
*
Corresponding author. Mailing address: National
Institutes of Health, Microbiology Service, Building 10, Room 2C-385,
Bethesda, MD 20892-1508. Phone: (301) 496-4433. Fax: (301) 402-1886. E-mail: gfahle{at}nih.gov.
Journal of Clinical Microbiology, October 2000, p. 3860-3863, Vol. 38, No. 10
0095-1137/00/$04.00+0
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