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Journal of Clinical Microbiology, October 2000, p. 3876-3878, Vol. 38, No. 10
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

VP4 and VP7 Genotyping by Reverse Transcription-PCR of Human Rotavirus in Mexican Children with Acute Diarrhea

Araceli Rodríguez Castillo, Andrés Velasco Villa, José Ernesto Ramírez González, Elvira Mayén Pimentel, Martín Melo Munguía, Benita Díaz de Jesús, Hiram Olivera Díaz, and Herlinda García Lozano*

Laboratorio de Rotavirus, Instituto Nacional de Diagnóstico y Referencia Epidemiológicos (INDRE), Secretaría de Salud, Mexico City, Mexico

Received 1 December 1999/Returned for modification 2 May 2000/Accepted 12 July 2000

Dual typing (VP4 and VP7) of rotavirus obtained from 257 Mexican children during three epidemiological seasons was performed by reverse transcription-PCR. The P1G1 genotype was the most prevalent (40%), followed by P1G3 (19%) and P2G2 (16%). Thirty-one specimens (12%) presented mixed infections, while some genotypes were not found. This is the first dual typing of isolates from diarrhea cases in Mexico.

* Corresponding author. Mailing address: Laboratorio de Rotavirus, INDRE-SSA, Carpio 470, Colonia Santo Tomas, C.P. 11340, Mexico. Phone: 525-3-41-49-53, ext. 234. Fax: 525-3-42-06-70. E-mail: indre{at}cenids.ssa.gob.mx.

Journal of Clinical Microbiology, October 2000, p. 3876-3878, Vol. 38, No. 10
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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