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Journal of Clinical Microbiology, October 2000, p. 3882-3886, Vol. 38, No. 10
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Evaluation of the Abbott LCx HIV-1 RNA Quantitative, a New Assay for Quantitative Determination of Human Immunodeficiency Virus Type 1 RNA

Nadia Zanchetta,1,* Giampiero Nardi,1 Loredana Tocalli,1 Lorenzo Drago,1 Carla Bossi,1 Francesco Renato Pulvirenti,2 Claudio Galli,2 and Maria Rita Gismondo1

Microbiology, "L. Sacco" Teaching Hospital, Milan,1 and Scientific Affairs, Abbott Diagnostics Division, Rome,2 Italy

Received 24 April 2000/Returned for modification 6 June 2000/Accepted 17 July 2000

A new quantitative reverse transcription (RT)-PCR assay for human immunodeficiency virus type 1 (HIV-1) RNA (Abbott LCx HIV RNA Quantitative assay) has been compared with the Organon NucliSens assay on 521 retrospective samples obtained from HIV-1-positive patients monitored during highly active antiretroviral therapy, 79 of whom were assayed also by the Chiron Quantiplex 3.0 system and on characterized panels. The LCx system showed a moderate correlation (r = 0.795) and gave higher results than the NucliSens system on 245 of 327 concordant positive samples, with similar sensitivity. Correlation with Quantiplex system results was higher (r = 0.943). LCx reproducibility was very good; the procedure was simple, well controlled, and rapid (up to 48 results in 7 h). The HIV RNA quantitative assay on the LCx system is suitable for routine use.

* Corresponding author. Mailing address: Microbiologia, Ospedale "Luigi Sacco," Via G. B. Grassi 74, 20175 Milano, Italy. Phone: 39-02-39042479. Fax: 39-02-39042313. E-mail: microbio{at}mailserver.unimi.it.

Journal of Clinical Microbiology, October 2000, p. 3882-3886, Vol. 38, No. 10
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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Copyright © 2000 by the American Society for Microbiology. All rights reserved.