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Journal of Clinical Microbiology, October 2000, p. 3896-3899, Vol. 38, No. 10
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Mycobacterium branderi from Both a Hand Infection and a Case of Pulmonary Disease

Joyce Wolfe,1,2 Christine Turenne,2,* Michelle Alfa,3 Godfrey Harding,3 Louise Thibert,4 and Amin Kabani1,2

National Reference Centre for Mycobacteriology, Bureau of Microbiology, Canadian Science Centre for Human and Animal Health,2 Clinical Microbiology, Health Sciences Centre,1 and St-Boniface General Hospital,3 Winnipeg, Manitoba, and Laboratoire de santé publique du Québec, Sainte-Anne-de-Bellevue, Québec,4 Canada

Received 3 April 2000/Returned for modification 16 May 2000/Accepted 22 July 2000

Mycobacterium branderi, a potential human pathogen first characterized in 1995, has been isolated from respiratory tract specimens. We report here a case in which M. branderi was the only organism isolated upon culture from a hand infection. This isolate, along with a second isolate from a bronchial specimen, was subjected to conventional identification tests for mycobacterial species. Further analysis by high-performance liquid chromatography (HPLC) of mycolic acids and 16S rRNA gene sequencing was performed, and the antibiotic susceptibility profile was determined for both strains. Biochemical tests and the HPLC pattern were consistent with that of M. branderi and M. celatum, which are very similar. The 16S rRNA gene sequence of both strains corresponded to that of M. branderi and enabled us to confidently differentiate this organism from other closely related species such as M. celatum. This contributes to a further understanding of the status of this species as a potential human pathogen as well as illustrating the need for molecular diagnostics as a complementary method for the identification of rare mycobacterial species.

* Corresponding author. Mailing address: National Reference Centre for Mycobacteriology, Bureau of Microbiology, Canadian Science Centre for Human and Animal Health, 1015 Arlington St., Winnipeg, Manitoba R3E 3R2, Canada. Phone: (204) 789-6081. Fax: (204) 789-2036. E-mail: cturenne{at}hc-sc.gc.ca.

Journal of Clinical Microbiology, October 2000, p. 3896-3899, Vol. 38, No. 10
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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