This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Goh, S. H.
Right arrow Articles by Hemmingsen, S. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Goh, S. H.
Right arrow Articles by Hemmingsen, S. M.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, November 2000, p. 3953-3959, Vol. 38, No. 11
0095-1137/00/$04.00+0

Identification of Enterococcus Species and Phenotypically Similar Lactococcus and Vagococcus Species by Reverse Checkerboard Hybridization to Chaperonin 60 Gene Sequences

Swee Han Goh,1,2,* Richard R. Facklam,3 Michelle Chang,1 Janet E. Hill,5 Gregory J. Tyrrell,4 Emma C. M. Burns,2 David Chan,2 Cheng He,2 Tazim Rahim,2 Carol Shaw,2 and Sean M. Hemmingsen5

Department of Pathology & Laboratory Medicine, The University of British Columbia,1 and Laboratory Services, British Columbia Centre for Disease Control Society,2 Vancouver, British Columbia, National Centre for Streptococcus, Edmonton, Alberta,4 and National Research Council Canada, Plant Biotechnology Institute, Saskatoon, Saskatchewan,5 Canada, and Centers for Disease Control and Prevention, Atlanta, Georgia3

Received 26 May 2000/Returned for modification 27 July 2000/Accepted 28 August 2000

Data from four recent studies (S. H. Goh et al., J. Clin. Microbiol. 36:2164-2166, 1998; S. H. Goh et al., J. Clin. Microbiol. 34:818-823, 1996; S. H. Goh et al., J. Clin. Microbiol. 35:3116-3121, 1997; A. Y. C. Kwok et al., Int. J. Syst. Bacteriol. 49:1181-1192, 1999) suggest that an approximately 600-bp region of the chaperonin 60 (Cpn60) gene, amplified by PCR with a single pair of degenerate primers, has utility as a potentially universal target for bacterial identification (ID). This Cpn60 gene ID method correctly identified isolates representative of numerous staphylococcal species and Streptococcus iniae, a human and animal pathogen. We report herein that this method enabled us to distinguish clearly between 17 Enterococcus species (Enterococcus asini, Enterococcus rattus, Enterococcus dispar, Enterococcus gallinarum, Enterococcus hirae, Enterococcus durans, Enterococcus cecorum, Enterococcus faecalis, Enterococcus mundtii, Enterococcus casseliflavus, Enterococcus faecium, Enterococcus malodoratus, Enterococcus raffinosus, Enterococcus avium, Enterococcus pseudoavium, Enterococcus new sp. strain Facklam, and Enterococcus saccharolyticus), and Vagococcus fluvialis, Lactococcus lactis, and Lactococcus garvieae. From 123 blind-tested samples, only two discrepancies were observed between the Facklam and Collins phenotyping method (R. R. Facklam and M. D. Collins, J. Clin. Microbiol. 27:731-734, 1989) and the Cpn60 ID method. In each case, the discrepancies were resolved in favor of the Cpn60 ID method. The species distributions of the 123 blind-tested isolates were Enterococcus new sp. strain Facklam (ATCC 700913), 3; E. asini, 1; E. rattus, 4; E. dispar, 2; E. gallinarum, 20; E. hirae, 9; E. durans, 9; E. faecalis, 12; E. mundtii, 3; E. casseliflavus, 8; E. faecium, 25; E. malodoratus, 3; E. raffinosus, 8; E. avium, 4; E. pseudoavium, 1; an unknown Enterococcus clinical isolate, sp. strain R871; Vagococcus fluvialis, 4; Lactococcus garvieae, 3; Lactococcus lactis, 3; Leuconostoc sp., 1; and Pediococcus sp., 1. The Cpn60 gene ID method, coupled with reverse checkerboard hybridization, is an effective method for the identification of Enterococcus and related organisms.

* Corresponding author. Mailing address: Dept. of Pathology & Laboratory Medicine, The University of British Columbia, and Laboratory Services, British Columbia Centre for Disease Control Society, 655W 12th Ave., Vancouver, British Columbia V5Z 4R4, Canada. Phone: (604) 660-6005. Fax: (604) 660-0403. E-mail: shgoh{at}interchange.ubc.ca.

Journal of Clinical Microbiology, November 2000, p. 3953-3959, Vol. 38, No. 11
0095-1137/00/$04.00+0


This article has been cited by other articles:

  • Haigh, J. C., Gerwing, V., Erdenebaatar, J., Hill, J. E. (2008). A NOVEL CLINICAL SYNDROME AND DETECTION OF ANAPLASMA OVIS IN MONGOLIAN REINDEER (RANGIFER TARANDUS). J Wildl Dis 44: 569-577 [Abstract] [Full Text]  
  • Blaiotta, G., Fusco, V., Ercolini, D., Aponte, M., Pepe, O., Villani, F. (2008). Lactobacillus Strain Diversity Based on Partial hsp60 Gene Sequences and Design of PCR-Restriction Fragment Length Polymorphism Assays for Species Identification and Differentiation. Appl. Environ. Microbiol. 74: 208-215 [Abstract] [Full Text]  
  • Menendez, A., Fernandez, L., Reimundo, P., Guijarro, J. A. (2007). Genes required for Lactococcus garvieae survival in a fish host. Microbiology 153: 3286-3294 [Abstract] [Full Text]  
  • Hill, J. E., Paccagnella, A., Law, K., Melito, P. L., Woodward, D. L., Price, L., Leung, A. H., Ng, L.-K., Hemmingsen, S. M., Goh, S. H. (2006). Identification of Campylobacter spp. and discrimination from Helicobacter and Arcobacter spp. by direct sequencing of PCR-amplified cpn60 sequences and comparison to cpnDB, a chaperonin reference sequence database.. J Med Microbiol 55: 393-399 [Abstract] [Full Text]  
  • Hung, W.-C., Tsai, J.-C., Hsueh, P.-R., Chia, J.-S., Teng, L.-J. (2005). Species identification of mutans streptococci by groESL gene sequence. J Med Microbiol 54: 857-862 [Abstract] [Full Text]  
  • Hill, J. E., Hemmingsen, S. M., Goldade, B. G., Dumonceaux, T. J., Klassen, J., Zijlstra, R. T., Goh, S. H., Van Kessel, A. G. (2005). Comparison of Ileum Microflora of Pigs Fed Corn-, Wheat-, or Barley-Based Diets by Chaperonin-60 Sequencing and Quantitative PCR. Appl. Environ. Microbiol. 71: 867-875 [Abstract] [Full Text]  
  • Tsai, J.-C., Hsueh, P.-R., Lin, H.-M., Chang, H.-J., Ho, S.-W., Teng, L.-J. (2005). Identification of Clinically Relevant Enterococcus Species by Direct Sequencing of groES and Spacer Region. J. Clin. Microbiol. 43: 235-241 [Abstract] [Full Text]  
  • Jackson, C. R., Fedorka-Cray, P. J., Barrett, J. B. (2004). Use of a Genus- and Species-Specific Multiplex PCR for Identification of Enterococci. J. Clin. Microbiol. 42: 3558-3565 [Abstract] [Full Text]  
  • Hill, J. E., Penny, S. L., Crowell, K. G., Goh, S. H., Hemmingsen, S. M. (2004). cpnDB: A Chaperonin Sequence Database. Genome Res 14: 1669-1675 [Abstract] [Full Text]  
  • Hoffmann, H., Roggenkamp, A. (2003). Population Genetics of the Nomenspecies Enterobacter cloacae. Appl. Environ. Microbiol. 69: 5306-5318 [Abstract] [Full Text]  
  • Lee, J.-H., Park, H.-S., Jang, W.-J., Koh, S.-E., Kim, J.-M., Shim, S.-K., Park, M.-Y., Kim, Y.-W., Kim, B.-J., Kook, Y.-H., Park, K.-H., Lee, S.-H. (2003). Differentiation of Rickettsiae by groEL Gene Analysis. J. Clin. Microbiol. 41: 2952-2960 [Abstract] [Full Text]  
  • Kwok, A. Y. C., Chow, A. W. (2003). Phylogenetic study of Staphylococcus and Macrococcus species based on partial hsp60 gene sequences. Int. J. Syst. Evol. Microbiol. 53: 87-92 [Abstract] [Full Text]  
  • Teng, L.-J., Hsueh, P.-R., Tsai, J.-C., Chen, P.-W., Hsu, J.-C., Lai, H.-C., Lee, C.-N., Ho, S.-W. (2002). groESL Sequence Determination, Phylogenetic Analysis, and Species Differentiation for Viridans Group Streptococci. J. Clin. Microbiol. 40: 3172-3178 [Abstract] [Full Text]  
  • Hill, J. E., Seipp, R. P., Betts, M., Hawkins, L., Van Kessel, A. G., Crosby, W. L., Hemmingsen, S. M. (2002). Extensive Profiling of a Complex Microbial Community by High-Throughput Sequencing. Appl. Environ. Microbiol. 68: 3055-3066 [Abstract] [Full Text]  
  • Brousseau, R., Hill, J. E., Prefontaine, G., Goh, S.-H., Harel, J., Hemmingsen, S. M. (2001). Streptococcus suis Serotypes Characterized by Analysis of Chaperonin 60 Gene Sequences. Appl. Environ. Microbiol. 67: 4828-4833 [Abstract] [Full Text]  
  • Teng, L.-J., Hsueh, P.-R., Wang, Y.-H., Lin, H.-M., Luh, K.-T., Ho, S.-W. (2001). Determination of Enterococcus faecalis groESL Full-Length Sequence and Application for Species Identification. J. Clin. Microbiol. 39: 3326-3331 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»