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Journal of Clinical Microbiology, November 2000, p. 4026-4033, Vol. 38, No. 11
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Molecular and Pathogenic Characterization of Borrelia burgdorferi Sensu Lato Isolates from Spain

Raquel Escudero,1 Marta Barral,2 Azucena Pérez,3 M. Mar Vitutia,4 Ana L. García-Pérez,2 Santos Jiménez,3 Ricela E. Sellek,1 and Pedro Anda1,*

Servicio de Bacteriología1 and Servicio de Parasitología,4 Centro Nacional de Microbiología, Instituto de Salud Carlos III, 28220 Majadahonda, Madrid, Servicio de Investigación y Mejora Agraria (AZTI-SIMA), Departamento de Agricultura, Gobierno Vasco, 48160 Derio, Vizcaya,2 and Consejería de Salud, Consumo y Bienestar Social del Gobierno de la Rioja, 26071 Logroño,3 Spain

Received 25 May 2000/Accepted 29 August 2000

Fifteen Borrelia burgdorferi sensu lato isolates from questing ticks and skin biopsy specimens from erythema migrans patients in three different areas of Spain were characterized. Four different genospecies were found (nine Borrelia garinii, including the two human isolates, three B. burgdorferi sensu stricto, two B. valaisiana, and one B. lusitaniae), showing a diverse spectrum of B. burgdorferi sensu lato species. B. garinii isolates were highly variable in terms of pulsed-field gel electrophoresis pattern and OspA serotype, with four of the seven serotypes described. One of the human isolates was OspA serotype 5, the same found in four of seven tick isolates. The second human isolate was OspA serotype 3, which was not present in ticks from the same area. Seven B. garinii isolates were able to disseminate through the skin of C3H/HeN mice and to cause severe inflammation of joints. One of the two B. valaisiana isolates also caused disease in mice. Only one B. burgdorferi sensu stricto isolate was recovered from the urinary bladder. One isolate each of B. valaisiana and B. lusitaniae were not able to disseminate through the skin of mice or to infect internal organs. In summary, there is substantial diversity in the species and in the pathogenicity of B. burgdorferi sensu lato in areas in northern Spain where Lyme disease is endemic.

* Corresponding author. Mailing address: Servicio de Bacteriología, Centro Nacional de Microbiología-Instituto de Salud Carlos III, 28220-Majadahonda, Madrid, Spain. Phone: (34) 91 509 7901. Fax: (34) 91 509 7966. E-mail: panda{at}isciii.es.

Journal of Clinical Microbiology, November 2000, p. 4026-4033, Vol. 38, No. 11
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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