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Journal of Clinical Microbiology, November 2000, p. 4114-4120, Vol. 38, No. 11
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Species-Specific Identification of Human Adenoviruses by a Multiplex PCR Assay

WanHong Xu, Mike C. McDonough, and Dean D. Erdman*

Respiratory and Enteric Viruses Branch, Division of Viral and Rickettsial Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia

Received 12 June 2000/Returned for modification 17 July 2000/Accepted 9 August 2000

A multiplex PCR assay was developed by using primers to the fiber gene that could differentiate human adenovirus (Ad) species A through F in a single amplification reaction. The assay correctly identified the species of all 49 recognized Ad prototype strains as well as 180 geographically and temporally diverse Ad field isolates. Ad serotype 6 (Ad6) (species C), Ad16 (species B), Ad31 (species A), and Ad40 and Ad41 (species F) could also be distinguished by amplicon size within each respective species. In comparison, a previously described Ad species-specific multiplex PCR assay that used primers to the Ad hexon gene gave equivocal results with several serotypes of species B, whereas our multiplex assay amplified all species B serotypes equally well. Our multiplex PCR assay will permit rapid, accurate, and cost-effective classification of Ad isolates.

* Corresponding author. Mailing address: Centers for Disease Control and Prevention, Mailstop G-09, 1600 Clifton Rd., NE, Atlanta, GA 30333. Phone: (404) 639-3727. Fax: (404) 639-1307. E-mail: dde1{at}cdc.gov.

Journal of Clinical Microbiology, November 2000, p. 4114-4120, Vol. 38, No. 11
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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