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Journal of Clinical Microbiology, November 2000, p. 4215-4218, Vol. 38, No. 11
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Detection of Legionella pneumophila Using a Real-Time PCR Hybridization Assay

A. L. Ballard,1 N. K. Fry,2 L. Chan,3 S. B. Surman,3 J. V. Lee,3 T. G. Harrison,2 and K. J. Towner1,*

Department of Microbiology and PHLS Laboratory, University Hospital,1 and PHLS Water and Environmental Microbiology Research Unit,3 Queen's Medical Centre, Nottingham NG7 2UH, and Respiratory and Systemic Infection Laboratory, PHLS Central Public Health Laboratory, London NW9 5HT,2 United Kingdom

Received 29 March 2000/Returned for modification 8 June 2000/Accepted 18 August 2000

A real-time PCR hybridization assay for Legionella pneumophila is described; the assay uses LightCycler (Idaho Technology) methodology to specifically detect 2.5 CFU/reaction, equivalent to 1,000 CFU/liter of starting water sample. The assay, including DNA extraction and confirmation of product identity, is completed within 90 min of receipt of a sample.

* Corresponding author. Mailing address: Department of Microbiology and PHLS Laboratory, University Hospital, Queen's Medical Centre, Nottingham NG7 2UH, United Kingdom. Phone: 44-115-9709163. Fax: 44-115-9422190. E-mail: Kevin.Towner{at}nottingham.ac.uk.

Journal of Clinical Microbiology, November 2000, p. 4215-4218, Vol. 38, No. 11
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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