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Journal of Clinical Microbiology, November 2000, p. 4219-4221, Vol. 38, No. 11
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Detection of Ehrlichia platys DNA in Brown Dog Ticks (Rhipicephalus sanguineus) in Okinawa Island, Japan

Hisashi Inokuma,1,2 Didier Raoult,2 and Philippe Brouqui2,*

Laboratory of Veterinary Internal Medicine, Faculty of Agriculture, Yamaguchi University, Yamaguchi, Japan,1 and Unité des Rickettsies, Faculté de Médecine, Université de la Méditerranée, Marseille Cédex 5, France2

Received 10 April 2000/Returned for modification 5 July 2000/Accepted 21 August 2000

Thirty-two Rhipicephalus sanguineus females collected from eight free-roaming dogs in Okinawa Island, Japan, were examined for ehrlichial DNA by 16S rRNA-based PCR and subsequent sequencing. Partial sequences of Ehrlichia platys 16S rRNA (678 to 679 bp) were detected in three ticks (9.4%) from two dogs. This is the first report of detection of E. platys in Japan, and also the first report of detection in ticks.

* Corresponding author. Mailing address: Unité des Rickettsies, Faculté de Médecine, 27 bd. Jean Moulin, 13385 Marseille Cédex 5, France. Phone: (33)-491-32-43-75. Fax: (33)-4-91-83-03-90. E-mail: Philippe.Brouqui{at}medecine.univ-mrs.fr.

Journal of Clinical Microbiology, November 2000, p. 4219-4221, Vol. 38, No. 11
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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