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Journal of Clinical Microbiology, November 2000, p. 4228-4232, Vol. 38, No. 11
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Detection of Extended-Spectrum beta -Lactamases in Klebsiellae with the Oxoid Combination Disk Method

Michael W. Carter, Karen J. Oakton, Marina Warner, and David M. Livermore*

Antibiotic Resistance Monitoring and Reference Laboratory, Central Public Health Laboratory, London NW9 5HT, United Kingdom

Received 19 June 2000/Returned for modification 1 August 2000/Accepted 21 August 2000

The Oxoid combination disk method for detecting extended-spectrum beta -lactamases (ESBLs) depends on comparing the inhibition zones of cefpodoxime (10-µg) and cefpodoxime-plus-clavulanate (10- plus 1-µg) disks. The presence of clavulanate enlarged the zones for all of 180 ESBL-producing klebsiellae by >= 5 mm, whereas zones for cefpodoxime-susceptible isolates and cefpodoxime-resistant isolates with AmpC and K1 beta -lactamases were enlarged by <= 1 mm. Good discrimination was achieved with either the NCCLS or the British disk method.


* Corresponding author. Mailing address: Antibiotic Resistance Monitoring and Reference Laboratory, Central Public Health Laboratory, 61 Colindale Ave., London NW9 5HT, United Kingdom. Phone: 44-208-200-4400. Fax: 44-208-358-3292. E-mail: DLivermore{at}phls.nhs.uk.


Journal of Clinical Microbiology, November 2000, p. 4228-4232, Vol. 38, No. 11
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.



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