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Journal of Clinical Microbiology, November 2000, p. 4228-4232, Vol. 38, No. 11
Antibiotic Resistance Monitoring and
Reference Laboratory, Central Public Health Laboratory, London NW9
5HT, United Kingdom
Received 19 June 2000/Returned for modification 1 August
2000/Accepted 21 August 2000
The Oxoid combination disk method for detecting extended-spectrum
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Detection of Extended-Spectrum
-Lactamases in
Klebsiellae with the Oxoid Combination Disk Method
-lactamases (ESBLs) depends on comparing the inhibition zones of
cefpodoxime (10-µg) and cefpodoxime-plus-clavulanate (10- plus
1-µg) disks. The presence of clavulanate enlarged the zones for all
of 180 ESBL-producing klebsiellae by
5 mm, whereas zones for
cefpodoxime-susceptible isolates and cefpodoxime-resistant isolates
with AmpC and K1
-lactamases were enlarged by
1 mm. Good
discrimination was achieved with either the NCCLS or the British disk method.
*
Corresponding author. Mailing address: Antibiotic
Resistance Monitoring and Reference Laboratory, Central Public Health
Laboratory, 61 Colindale Ave., London NW9 5HT, United Kingdom. Phone:
44-208-200-4400. Fax: 44-208-358-3292. E-mail:
DLivermore{at}phls.nhs.uk.
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