This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Carter, M. W.
Right arrow Articles by Livermore, D. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Carter, M. W.
Right arrow Articles by Livermore, D. M.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, November 2000, p. 4228-4232, Vol. 38, No. 11
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Detection of Extended-Spectrum beta -Lactamases in Klebsiellae with the Oxoid Combination Disk Method

Michael W. Carter, Karen J. Oakton, Marina Warner, and David M. Livermore*

Antibiotic Resistance Monitoring and Reference Laboratory, Central Public Health Laboratory, London NW9 5HT, United Kingdom

Received 19 June 2000/Returned for modification 1 August 2000/Accepted 21 August 2000

The Oxoid combination disk method for detecting extended-spectrum beta -lactamases (ESBLs) depends on comparing the inhibition zones of cefpodoxime (10-µg) and cefpodoxime-plus-clavulanate (10- plus 1-µg) disks. The presence of clavulanate enlarged the zones for all of 180 ESBL-producing klebsiellae by >= 5 mm, whereas zones for cefpodoxime-susceptible isolates and cefpodoxime-resistant isolates with AmpC and K1 beta -lactamases were enlarged by <= 1 mm. Good discrimination was achieved with either the NCCLS or the British disk method.

* Corresponding author. Mailing address: Antibiotic Resistance Monitoring and Reference Laboratory, Central Public Health Laboratory, 61 Colindale Ave., London NW9 5HT, United Kingdom. Phone: 44-208-200-4400. Fax: 44-208-358-3292. E-mail: DLivermore{at}phls.nhs.uk.

Journal of Clinical Microbiology, November 2000, p. 4228-4232, Vol. 38, No. 11
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Torpdahl, M., Hammerum, A. M., Zachariasen, C., Nielsen, E. M. (2009). Detection of qnr genes in Salmonella isolated from humans in Denmark. J Antimicrob Chemother 63: 406-408 [Full Text]  
  • Navon-Venezia, S., Chmelnitsky, I., Leavitt, A., Carmeli, Y. (2008). Dissemination of the CTX-M-25 family {beta}-lactamases among Klebsiella pneumoniae, Escherichia coli and Enterobacter cloacae and identification of the novel enzyme CTX-M-41 in Proteus mirabilis in Israel. J Antimicrob Chemother 62: 289-295 [Abstract] [Full Text]  
  • Warren, R. E., Ensor, V. M., O'Neill, P., Butler, V., Taylor, J., Nye, K., Harvey, M., Livermore, D. M., Woodford, N., Hawkey, P. M. (2008). Imported chicken meat as a potential source of quinolone-resistant Escherichia coli producing extended-spectrum {beta}-lactamases in the UK. J Antimicrob Chemother 61: 504-508 [Abstract] [Full Text]  
  • Beceiro, A., Fernandez-Cuenca, F., Ribera, A., Martinez-Martinez, L., Pascual, A., Vila, J., Rodriguez-Bano, J., Cisneros, J. M., Pachon, J., Bou, G., on behalf of the Spanish Group for Nosocomial Infe, (2008). False extended-spectrum {beta}-lactamase detection in Acinetobacter spp. due to intrinsic susceptibility to clavulanic acid. J Antimicrob Chemother 61: 301-308 [Abstract] [Full Text]  
  • Harish, B. N., Menezes, G. A., Shekatkar, S., Parija, S. C. (2007). Extended-spectrum {beta}-lactamase-producing Klebsiella pneumoniae from blood culture. J Med Microbiol 56: 999-1000 [Full Text]  
  • Birkett, C. I., Ludlam, H. A., Woodford, N., Brown, D. F. J., Brown, N. M., Roberts, M. T. M., Milner, N., Curran, M. D. (2007). Real-time TaqMan PCR for rapid detection and typing of genes encoding CTX-M extended-spectrum {beta}-lactamases. J Med Microbiol 56: 52-55 [Abstract] [Full Text]  
  • Ensor, V. M., Shahid, M., Evans, J. T., Hawkey, P. M. (2006). Occurrence, prevalence and genetic environment of CTX-M {beta}-lactamases in Enterobacteriaceae from Indian hospitals. J Antimicrob Chemother 58: 1260-1263 [Abstract] [Full Text]  
  • Jiang, X., Zhang, Z., Li, M., Zhou, D., Ruan, F., Lu, Y. (2006). Detection of Extended-Spectrum {beta}-Lactamases in Clinical Isolates of Pseudomonas aeruginosa.. Antimicrob. Agents Chemother. 50: 2990-2995 [Abstract] [Full Text]  
  • Spanu, T., Sanguinetti, M., Tumbarello, M., D'Inzeo, T., Fiori, B., Posteraro, B., Santangelo, R., Cauda, R., Fadda, G. (2006). Evaluation of the New VITEK 2 Extended-Spectrum Beta-Lactamase (ESBL) Test for Rapid Detection of ESBL Production in Enterobacteriaceae Isolates.. J. Clin. Microbiol. 44: 3257-3262 [Abstract] [Full Text]  
  • Thomas, K., Weinbren, M. J., Warner, M., Woodford, N., Livermore, D. (2006). Activity of mecillinam against ESBL producers in vitro. J Antimicrob Chemother 57: 367-368 [Full Text]  
  • Chmelnitsky, I., Carmeli, Y., Leavitt, A., Schwaber, M. J., Navon-Venezia, S. (2005). CTX-M-2 and a New CTX-M-39 Enzyme Are the Major Extended-Spectrum Beta-Lactamases in Multiple Escherichia coli Clones Isolated in Tel Aviv, Israel. Antimicrob. Agents Chemother. 49: 4745-4750 [Abstract] [Full Text]  
  • Paterson, D. L., Bonomo, R. A. (2005). Extended-Spectrum {beta}-Lactamases: a Clinical Update. Clin. Microbiol. Rev. 18: 657-686 [Abstract] [Full Text]  
  • Livermore, D. M., Hawkey, P. M. (2005). CTX-M: changing the face of ESBLs in the UK. J Antimicrob Chemother 56: 451-454 [Abstract] [Full Text]  
  • Navon-Venezia, S., Leavitt, A., Ben-Ami, R., Aharoni, Y., Schwaber, M. J., Schwartz, D., Carmeli, Y. (2005). Evaluation of an Accelerated Protocol for Detection of Extended-Spectrum {beta}-Lactamase-Producing Gram-Negative Bacilli from Positive Blood Cultures. J. Clin. Microbiol. 43: 439-441 [Abstract] [Full Text]  
  • De Gheldre, Y., Avesani, V., Berhin, C., Delmee, M., Glupczynski, Y. (2003). Evaluation of Oxoid combination discs for detection of extended-spectrum {beta}-lactamases. J Antimicrob Chemother 52: 591-597 [Abstract] [Full Text]  
  • Weldhagen, G. F., Poirel, L., Nordmann, P. (2003). Ambler Class A Extended-Spectrum {beta}-Lactamases in Pseudomonas aeruginosa: Novel Developments and Clinical Impact. Antimicrob. Agents Chemother. 47: 2385-2392 [Full Text]  
  • Barry, J., Brown, A., Ensor, V., Lakhani, U., Petts, D., Warren, C., Winstanley, T. (2003). Comparative evaluation of the VITEK 2 Advanced Expert System (AES) in five UK hospitals. J Antimicrob Chemother 51: 1191-1202 [Abstract] [Full Text]  
  • Sanguinetti, M., Posteraro, B., Spanu, T., Ciccaglione, D., Romano, L., Fiori, B., Nicoletti, G., Zanetti, S., Fadda, G. (2003). Characterization of Clinical Isolates of Enterobacteriaceae from Italy by the BD Phoenix Extended-Spectrum {beta}-Lactamase Detection Method. J. Clin. Microbiol. 41: 1463-1468 [Abstract] [Full Text]  
  • Bradford, P. A. (2001). Extended-Spectrum {beta}-Lactamases in the 21st Century: Characterization, Epidemiology, and Detection of This Important Resistance Threat. Clin. Microbiol. Rev. 14: 933-951 [Abstract] [Full Text]  
  • Randegger, C. C., Hächler, H. (2001). Real-Time PCR and Melting Curve Analysis for Reliable and Rapid Detection of SHV Extended-Spectrum {beta}-Lactamases. Antimicrob. Agents Chemother. 45: 1730-1736 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»