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Journal of Clinical Microbiology, November 2000, p. 4242-4245, Vol. 38, No. 11
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Improved Pulsed-Field Gel Electrophoresis for Typing Vancomycin-Resistant Enterococci

Durmishkhan Turabelidze,1 Mamuka Kotetishvili,1,2 Arnold Kreger,1 J. Glenn Morris Jr.,1 and Alexander Sulakvelidze1,*

Division of Molecular Epidemiology, Department of Epidemiology and Preventive Medicine, University of Maryland School of Medicine, Baltimore, Maryland 21201,1 and the Kanchaveli Institute of Plant Protection, Tbilisi, Georgia 3800772

Received 25 April 2000/Returned for modification 28 June 2000/Accepted 1 September 2000

A rapid protocol for subtyping vancomycin-resistant enterococci by pulsed-field gel electrophoresis is reported. The procedure is simple and potentially cost-effective and allows reproducible subtyping of the strains in approximately 1 day.

* Corresponding author. Mailing address: Division of Molecular Epidemiology, Department of Epidemiology and Preventive Medicine, University of Maryland School of Medicine, MSTF Bldg., Room 9-34, 10 S. Pine St., Baltimore, MD 21201. Phone: (410) 706-4587. Fax: (410) 706-4581. E-mail: asulakve{at}medicine.umaryland.edu.

Journal of Clinical Microbiology, November 2000, p. 4242-4245, Vol. 38, No. 11
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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