This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kong, F.
Right arrow Articles by Gilbert, G. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kong, F.
Right arrow Articles by Gilbert, G. L.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, November 2000, p. 4256-4259, Vol. 38, No. 11
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Rapid-Cycle PCR for Detection and Typing of Mycoplasma pneumoniae in Clinical Specimens

Fanrong Kong,1 Susanna Gordon,1 and Gwendolyn L. Gilbert1,2,*

Centre for Infectious Diseases and Microbiology, Institute of Clinical Pathology and Medical Research, Westmead Hospital,1 and National Centre for Immunisation Research and Surveillance of Vaccine Preventable Diseases, Royal Alexandra Hospital for Children,2 Westmead, New South Wales 2145, Australia

Received 3 May 2000/Returned for modification 10 July 2000/Accepted 8 September 2000

We designed several new primers and modified previously described species- and type-specific primers targeting the Mycoplasma pneumoniae P1 adhesin gene. Optimized thermal profiles allowed one-step or nested PCR to be completed in less than 1 h. In 10 patients with pneumonia, M. pneumoniae type 1 was identified in 3 and type 2 in 7.

* Corresponding author. Mailing address: Centre for Infectious Diseases and Microbiology, Institute of Clinical Pathology and Medical Research, Westmead Hospital, Darcy Rd., Westmead, New South Wales, 2145, Australia. Phone: (612) 9845 6255. Fax: (612) 9893 8659. E-mail: lyng{at}icpmr.wsahs.nsw.gov.au.

Journal of Clinical Microbiology, November 2000, p. 4256-4259, Vol. 38, No. 11
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Dumke, R., Luck, P. C., Noppen, C., Schaefer, C., von Baum, H., Marre, R., Jacobs, E. (2006). Culture-Independent Molecular Subtyping of Mycoplasma pneumoniae in Clinical Samples.. J. Clin. Microbiol. 44: 2567-2570 [Abstract] [Full Text]  
  • Waites, K. B., Talkington, D. F. (2004). Mycoplasma pneumoniae and Its Role as a Human Pathogen. Clin. Microbiol. Rev. 17: 697-728 [Abstract] [Full Text]  
  • Wang, H., Kong, F., Jelfs, P., James, G., Gilbert, G. L. (2004). Simultaneous Detection and Identification of Common Cell Culture Contaminant and Pathogenic Mollicutes Strains by Reverse Line Blot Hybridization. Appl. Environ. Microbiol. 70: 1483-1486 [Abstract] [Full Text]  
  • Loens, K., Ursi, D., Goossens, H., Ieven, M. (2003). Molecular Diagnosis of Mycoplasma pneumoniae Respiratory Tract Infections. J. Clin. Microbiol. 41: 4915-4923 [Full Text]  
  • Kong, F., Gowan, S., Martin, D., James, G., Gilbert, G. L. (2002). Molecular Profiles of Group B Streptococcal Surface Protein Antigen Genes: Relationship to Molecular Serotypes. J. Clin. Microbiol. 40: 620-626 [Abstract] [Full Text]  
  • Kong, F., Gowan, S., Martin, D., James, G., Gilbert, G. L. (2002). Serotype Identification of Group B Streptococci by PCR and Sequencing. J. Clin. Microbiol. 40: 216-226 [Abstract] [Full Text]  
  • Kong, F., James, G., Gordon, S., Zelynski, A., Gilbert, G. L. (2001). Species-Specific PCR for Identification of Common Contaminant Mollicutes in Cell Culture. Appl. Environ. Microbiol. 67: 3195-3200 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»