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Journal of Clinical Microbiology, November 2000, p. 4274-4276, Vol. 38, No. 11
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Is Chlamydia pneumoniae Present in Brain Lesions of Patients with Multiple Sclerosis?

Margaret R. Hammerschlag,1 Zheng Ke,2 Fengmin Lu,2 Patricia Roblin,1 Jens Boman,3 and Bernadette Kalman2,*

Chlamydia Research Laboratory, Department of Pediatrics, Division of Infectious Diseases, SUNY Health Science Center at Brooklyn, Brooklyn, New York1; Department of Neurology, MCP Hahnemann University, Philadelphia, Pennsylvania2; and Department of Virology, Umeå University, Umeå, Sweden3

Received 11 July 2000/Returned for modification 9 August 2000/Accepted 22 August 2000

We investigated the presence of Chlamydia pneumoniae in 81 normal and pathological specimens obtained from postmortem brain tissues of patients with multiple sclerosis and with other neurological or nonneurological diseases. The assays used included PCR amplification of all DNA samples in the initial study. Culture and a second PCR amplification of the organism in a subset of 19 brain specimens were also performed in two separate laboratories. All results were negative. Thus, this study on a large number of brain tissues suggests that C. pneumoniae is not involved in inflammatory demyelination.

* Corresponding author. Mailing address: Department of Neurology, MS 423, MCP Hahnemann University, 245 North 15th St., Philadelphia, PA 19102. Phone: (215) 762-1898. Fax: (215) 762-3161. E-mail: bernadette.kalman{at}drexel.edu.

Journal of Clinical Microbiology, November 2000, p. 4274-4276, Vol. 38, No. 11
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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