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Journal of Clinical Microbiology, December 2000, p. 4517-4522, Vol. 38, No. 12
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Conservation of Type-Specific B-Cell Epitopes of Glycoprotein G in Clinical Herpes Simplex Virus Type 2 Isolates

Jan-Åke Liljeqvist,* Bo Svennerholm, and Tomas Bergström

Department of Virology, Göteborg University, S-413 46 Göteborg, Sweden

Glycoprotein G (gG-2) of herpes simplex virus type 2 (HSV-2) is cleaved to a secreted amino-terminal portion and to a cell-associated, heavily O-glycosylated carboxy-terminal portion that constitutes the mature gG-2 (mgG-2). The mgG-2 protein is commonly used as a type-specific antigen in the serodiagnosis of HSV-2 infection. As the amino acid sequence variability of mgG-2 in clinical isolates may affect the performance of such assays, the gG-2 gene was sequenced from 15 clinical HSV-2 isolates. Few mutations were identified, and these were mostly localized outside the epitope regions described earlier. Five isolates were identical to different laboratory strains, indicating that the gG-2 gene is highly conserved over time. In the search for HSV-2 isolates harboring mutations within the immunodominant region of mgG-2, a pool of 2,400 clinical HSV-2 isolates was tested for reactivity with two anti-mgG-2 monoclonal antibodies (MAbs). Ten MAb escape HSV-2 mutants, which all harbored structurally restricted single- or dual-point mutations within the respective epitopes explaining the loss of binding, were identified. Sera from corresponding patients were reactive to mgG-2, as well as to a peptide representing the immunodominant region, suggesting that the point mutations detected did not diminish seroreactivity to mgG-2. The conservation of the gG-2 gene reported here further supports the use of mgG-2 as a type-specific antigen in the diagnosis of HSV-2 infections.

* Corresponding author. Mailing address: Department of Virology, Göteborg University, Guldhedsgatan 10 B, S-413 46 Göteborg, Sweden. Phone: 46 31 3424657. Fax: 46 31 3424960. E-mail: jan-ake.liljeqvist{at}microbio.gu.se.

Journal of Clinical Microbiology, December 2000, p. 4517-4522, Vol. 38, No. 12
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Norberg, P., Kasubi, M. J., Haarr, L., Bergstrom, T., Liljeqvist, J.-A. (2007). Divergence and Recombination of Clinical Herpes Simplex Virus Type 2 Isolates. J. Virol. 81: 13158-13167 [Abstract] [Full Text]  
  • Tunback, P., Bergstrom, T., Lowhagen, G.-B., Hoebeke, J., Liljeqvist, J.-A. (2005). Type-specific reactivity of anti-glycoprotein G antibodies from herpes simplex virus-infected individuals is maintained by single or dual type-specific residues. J. Gen. Virol. 86: 247-251 [Abstract] [Full Text]  
  • Norberg, P., Bergstrom, T., Rekabdar, E., Lindh, M., Liljeqvist, J.-A. (2004). Phylogenetic Analysis of Clinical Herpes Simplex Virus Type 1 Isolates Identified Three Genetic Groups and Recombinant Viruses. J. Virol. 78: 10755-10764 [Abstract] [Full Text]  
  • Rekabdar, E., Tunback, P., Liljeqvist, J.-A., Lindh, M., Bergstrom, T. (2002). Dichotomy of Glycoprotein G Gene in Herpes Simplex Virus Type 1 Isolates. J. Clin. Microbiol. 40: 3245-3251 [Abstract] [Full Text]  
  • Ikoma, M., Liljeqvist, J.-A., Groen, J., Glazenburg, K. L., The, T. H., Welling-Wester, S. (2002). Use of a Fragment of Glycoprotein G-2 Produced in the Baculovirus Expression System for Detecting Herpes Simplex Virus Type 2-Specific Antibodies. J. Clin. Microbiol. 40: 2526-2532 [Abstract] [Full Text]  


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