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Journal of Clinical Microbiology, December 2000, p. 4554-4559, Vol. 38, No. 12
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Comparative Genotyping of Candida albicans Bloodstream and Nonbloodstream Isolates at a Polymorphic Microsatellite Locus

Frédéric Dalle,1,2 Norelie Franco,3 José Lopez,1,2 Odile Vagner,1,2 Denis Caillot,4 Pascal Chavanet,2,5 Bernadette Cuisenier,1,2 Serge Aho,6 Sarab Lizard,3 and Alain Bonnin1,2,*

Laboratoire de Parasitologie et Mycologie,1 Laboratoire de Microbiologie Médicale et Moléculaire,2 Service d'Hématologie Clinique,4 Service des Maladies Infectieuses et Tropicales,5 and Service d'Epidémiologie et d'Hygiène Hospitalière,6 CHU et Faculté de Médecine, and Laboratoire de Génétique Moléculaire, Centre George François Leclerc,3 Dijon, France

Received 26 June 2000/Returned for modification 9 August 2000/Accepted 21 September 2000

Molecular typing studies have shown that the predominant form of reproduction of Candida albicans is clonal and that, in a majority of situations, persistent or recurrent infections are due to a unique strain. Characterization of distinct subpopulations and correlation with clinical features may thus be important to understanding the pathogenesis of candidiasis. In a clonal model, a unique polymorphic marker may identify populations with different biological properties. We therefore compared 48 bloodstream isolates and 48 nonbloodstream matched strains of C. albicans at the elongation factor 3-encoding gene (CEF3) polymorphic microsatellite locus of C. albicans. Sizing of the alleles was performed by automated capillary electrophoresis. A new, 137-bp allele was characterized, and seven nondescribed combinations were observed, resulting in 15 and 11 distinct CEF3 profiles in bloodstream and control strains, respectively. Genotypes 126-135, 130-136, and 131-131 accounted for 60.4% of both bloodstream and control strains. Four bloodstream isolates but no control strains displayed the 135-135 combination. None of the other genotypes was present at an increased frequency in bloodstream isolates. Bloodstream and nonbloodstream strains of C. albicans thus have a heterogeneous structure at the CEF3 locus, with three major and multiple minor allelic combinations.

* Corresponding author. Mailing address: Laboratoire de Parasitologie et Mycologie, Hôpital du Bocage, 21034 Dijon Cedex, France. Phone: 33 (0)3 80 29 36 03 or 33 (0)3 80 29 35 23. Fax: 33 (0)3 80 29 32 80. E-mail: alain.bonnin{at}chu-dijon.fr.

Journal of Clinical Microbiology, December 2000, p. 4554-4559, Vol. 38, No. 12
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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