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Journal of Clinical Microbiology, December 2000, p. 4560-4568, Vol. 38, No. 12
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Genomic and Phylogenetic Analysis of Hepatitis C Virus Isolates from Argentine Patients: a Six-Year Retrospective Study

J. F. Quarleri,1 B. H. Robertson,2 V. L. Mathet,1 M. Feld,1 L. Espínola,1 M. P. Requeijo,3 O. Mandó,3 G. Carballal,3 and J. R. Oubiña1,*

Laboratorio de Hepatitis Virales, Departamento Microbiología, Facultad de Medicina, Universidad de Buenos Aires,1 and CEMIC,3 Buenos Aires, Argentina, and Centers for Disease Control and Prevention, Atlanta, Georgia2

Received 15 November 1999/Returned for modification 30 June 2000/Accepted 7 September 2000

Typing of hepatitis C virus (HCV) isolates from Argentine patients was performed by using different methodologies in a population of 243 patients. HCV subtype was assigned based upon restriction fragment length polymorphism (RFLP). HCV RNA genomes obtained from serum samples were classified as belonging to clade 1 (53.5%), 2 (23.0%), or 3 (8.6%); 14.8% of samples showed HCV mixed infections, more frequently implying different subtypes within the same clade. In addition to RFLP typing, phylogenetic relatedness among sequences from both 5' untranslated region (n = 50) and nonstructural 5B coding region (n = 15) was established.

* Corresponding author. Mailing address: Dto. Microbiología, Fac. de Medicina, UBA, Paraguay 2155, Piso 11, (1121) Buenos Aires, Argentina. Phone: 54-11-4508-3689. Fax: 54-11-4508-3705. E-mail: joubina{at}fmed.uba.ar.

Journal of Clinical Microbiology, December 2000, p. 4560-4568, Vol. 38, No. 12
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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