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Journal of Clinical Microbiology, December 2000, p. 4580-4585, Vol. 38, No. 12
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Fluorescent Amplified-Fragment Length Polymorphism Genotyping of Neisseria meningitidis Identifies Clones Associated with Invasive Disease

Jonathan N. Goulding,1 John V. Hookey,1 John Stanley,1 Will Olver,2 Keith R. Neal,3 Dlawer A. A. Ala'Aldeen,2 and Catherine Arnold1,*

Molecular Biology Unit, SBVL, Central Public Health Laboratory, London NW9 5HT,1 and Meningococcal Research Group, Division of Microbiology,2 and Department of Public Health Medicine and Epidemiology,3 Queen's Medical Centre, Nottingham, United Kingdom

Received 12 May 2000/Returned for modification 10 July 2000/Accepted 3 October 2000

Fluorescent amplified-fragment length polymorphism (FAFLP), a genotyping technique with phylogenetic significance, was applied to 123 isolates of Neisseria meningitidis. Nine of these were from an outbreak in a British university; 9 were from a recent outbreak in Pontypridd, Glamorgan; 15 were from sporadic cases of meningococcal disease; 26 were from the National Collection of Type Cultures; 58 were carrier isolates from Ironville, Derbyshire; 1 was a disease isolate from Ironville; and five were representatives of invasive clones of N. meningitidis. FAFLP analysis results were compared with previously published multilocus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE) results. FAFLP was able to identify hypervirulent, hyperendemic lineages (invasive clones) of N. meningitidis as well as did MLST. PFGE did not discriminate between two strains from the outbreak that were classified as similar but distinct by FAFLP. The results suggest that high resolution of N. meningitidis for outbreak and other epidemiological analyses is more cost efficient by FAFLP than by sequencing procedures.

* Corresponding author. Mailing address: Molecular Biology Unit, SBVL, Central Public Health Laboratory, 61 Colindale Ave., London NW9 5HT, United Kingdom. Phone: 44 20 8200 4400. Fax: 44 20 8200 1569. E-mail: carnold{at}phls.nhs.uk.

Journal of Clinical Microbiology, December 2000, p. 4580-4585, Vol. 38, No. 12
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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