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Journal of Clinical Microbiology, December 2000, p. 4599-4603, Vol. 38, No. 12
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Evaluation of Etest for Susceptibility Testing of Multidrug-Resistant Isolates of Mycobacterium tuberculosis†

Manzour Hernando Hazbón,1,* Maria del Socorro Orozco,1 Luz Angela Labrada,1 Rafael Tovar,1 Kristen A. Weigle,2 and Audrey Wanger3

Centro Internacional de Entrenamiento e Investigaciones Médicas (CIDEIM), Cali, Colombia1; Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina2; and Department of Pathology and Laboratory Medicine, University of Texas Medical School, Houston, Texas3

Received 7 July 2000/Returned for modification 4 September 2000/Accepted 27 September 2000

To prescribe effective treatment schemes for patients with tuberculosis, more-efficient susceptibility testing techniques for Mycobacterium tuberculosis are needed, especially in regions with multidrug resistance. Etest (AB BIODISK, Solna, Sweden) is a simple technique that provides quantitative drug susceptibility results for M. tuberculosis in 5 to 10 days from a culture grown at low cost. The performance of Etest was compared to that of the reference proportion method, using 95 M. tuberculosis clinical isolates of which 42.1% (40 of 95) were resistant to at least one antibiotic by the reference method. Overall agreement between Etest and the reference method was 98.9% (94 of 95) for detection of multidrug resistance; for resistance to individual drugs, agreement was 97.9% (93 of 95) for rifampin, 96.0% (92 of 95) for ethambutol, 94.7% (90 of 95) for isoniazid, and 85.3% (81 of 95) for streptomycin. This study supports the utility of Etest for timely detection of drug resistance in M. tuberculosis and for use in tuberculosis control programs.

* Corresponding author. Mailing address: CIDEIM. Avenida 1 norte No. 3-03. Cali, Valle, Colombia. Phone: (57 2) 668 21 64. Fax: (57 2) 667 29 89. E-mail: molecular{at}cideim.org.co.

dagger Dedicated to the memory of Luis Ernesto Giraldo.

Journal of Clinical Microbiology, December 2000, p. 4599-4603, Vol. 38, No. 12
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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