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Journal of Clinical Microbiology, December 2000, p. 4614-4615, Vol. 38, No. 12
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Evaluation of Random Amplified Polymorphic DNA Typing of Pseudomonas aeruginosa

Maureen Campbell, Eshwar Mahenthiralingam,dagger and David P. Speert*

Division of Infectious and Immunological Diseases, Department of Pediatrics, University of British Columbia, and Children's and Women's Health Centre of British Columbia, Vancouver, British Columbia V5Z 4H4, Canada

Received 7 March 2000/Returned for modification 18 August 2000/Accepted 21 September 2000

A method for distinguishing among Pseudomonas aeruginosa strains using random amplified polymorphic DNA (RAPD) typing was evaluated for reproducibility and discriminatory power. A total of 200 isolates, blinded in triplicate, were evaluated by RAPD. All 600 samples were typeable; 197 of 200 isolates gave identical results on all three occasions, and 131 distinct RAPD types were identified.

* Corresponding author. Mailing address: B.C. Research, 950 West 28th Ave., Rm. 377, Vancouver, BC V5Z 4H4 Canada. Phone: 604-875-2438. Fax: 604-875-2226. E-mail: speert{at}interchange.ubc.ca.

dagger Present address: Cardiff School of Biosciences, Cardiff University, Cardiff, Wales C17 3T1, United Kingdom.

Journal of Clinical Microbiology, December 2000, p. 4614-4615, Vol. 38, No. 12
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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