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Journal of Clinical Microbiology, December 2000, p. 4626-4628, Vol. 38, No. 12
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Tween 80 Opacity Test Responses of Various Candida Species

Malcolm Slifkin*

Microbiology Section, Department of Pathology and Laboratory Medicine, Allegheny General Hospital, Pittsburgh, Pennsylvania 15212

Received 19 May 2000/Returned for modification 9 August 2000/Accepted 22 September 2000

A total of 111 Candida isolates representing 11 species were examined for their respective responses to a Tween 80 opacity test. The strains of Candida albicans and C. tropicalis that were examined produced an opacity response around their colonies at 2 to 3 days postinoculation. A second group of Candida species yielded a halo around their colonies at 8 to 10 days postinoculation. The remaining Candida species did not produce a positive test response through 10 days postinoculation. The strains of C. dubliniensis were easily differentiated from strains of C. albicans by this test. The Tween 80 opacity test is simple and economical to prepare and is easy to interpret.

* Mailing address: Allegheny General Hospital, 320 East North Ave., Pittsburgh, PA 15212. Phone: (412) 359-3529. Fax: (412) 359-3860. E-mail: Mslifkin{at}wpahs.org.

Journal of Clinical Microbiology, December 2000, p. 4626-4628, Vol. 38, No. 12
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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