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Journal of Clinical Microbiology, December 2000, p. 4629-4632, Vol. 38, No. 12
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

PCR Detection and Evidence of Shedding of Porcine Circovirus Type 2 in Boar Semen

Renée Larochelle,1,* Andrzej Bielanski,2 Peter Müller,1 and Ronald Magar1

Laboratoire d'Hygiène Vétérinaire et Alimentaire, Agence Canadienne d'Inspection des Aliments, Saint-Hyacinthe, Québec,1 and Animal Diseases Research Institute, Canadian Food Inspection Agency, Nepean, Ontario,2 Canada

Received 15 June 2000/Returned for modification 25 August 2000/Accepted 29 September 2000

An experimental study was conducted to evaluate the potential presence of porcine circovirus type 2 (PCV2) in the semen of infected boars. Four mature boars were inoculated intranasally with PCV2 isolate LHVA-V53 propagated on PK15 cells. Two boars inoculated with the supernatant of noninfected PK15 cells were kept as controls. Serum samples were collected from all boars at 4, 7, 11, 13, 18, 21, 25, 28, 35, and 55 days postinoculation (dpi) and from the four PCV2-infected boars at 90 dpi. Samples were tested for the presence of antibodies to PCV2 by an indirect immunofluorescence assay and for the presence of PCV2 DNA by PCR and nested PCR. Semen samples were collected from all six boars at 5, 8, 11, 13, 18, 21, 25, 28, 33, and 47 dpi and tested for the presence of PCV2 DNA by a nested PCR assay. Antibodies to PCV2 could be detected as early as 11 dpi in one boar, and all four infected boars were found positive for PCV2 antibodies by 18 dpi. Thereafter all infected boars remained positive for antibodies to PCV2 until 90 dpi. Analysis of serum samples by nested PCR demonstrated the presence of PCV2 DNA as early as 4 dpi in three of four infected boars. Serum samples from all infected boars were positive for PCV2 DNA from 11 dpi until 35 dpi but were negative at 90 dpi. PCV2 DNA was detected as soon as 5 dpi in the semen of two infected boars and intermittently thereafter in the semen of all four infected boars. The semen of two infected boars was positive for PCV2 DNA at 47 dpi. Following infection, PCV2 DNA can be detected in semen concurrently with the presence of PCV2 DNA and antibodies in the serum. The present study suggests that PCV2 may be shed intermittently in the semen of infected boars.

* Corresponding author. Mailing address: Laboratoire d'Hygiène Vétérinaire et Alimentaire, Agence Canadienne d'Inspection des Aliments, 3400 Casavant Ouest, Saint-Hyacinthe, Québec, Canada J2S 8E3. Phone: (450) 773-7730. Fax: (450) 773-8152. E-mail: larocheller{at}em.agr.ca.

Journal of Clinical Microbiology, December 2000, p. 4629-4632, Vol. 38, No. 12
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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