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Journal of Clinical Microbiology, December 2000, p. 4637-4639, Vol. 38, No. 12
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Comparison of Human Immunodeficiency Virus Type 1 RNA Sequence Heterogeneity in Cerebrospinal Fluid and Plasma

Yi-Wei Tang,1,2,* Joe T.-J. Huong,3 Robert M. Lloyd Jr.,3 Paul Spearman,4,5 and David W. Haas1,5

Departments of Medicine,1 Pathology,2 Pediatrics,4 and Microbiology and Immunology,5 Vanderbilt University School of Medicine, Nashville, Tennessee 37232, and Applied Sciences, Inc., Norcross, Georgia 300923

Received 26 June 2000/Returned for modification 10 August 2000/Accepted 7 September 2000

The source of human immunodeficiency virus type 1 (HIV-1) RNA in cerebrospinal fluid (CSF) during HIV-1 infection is uncertain. The sequence heterogeneity of HIV-1 RNA in simultaneous CSF and plasma samples was characterized for five patients at the baseline and during the first week of antiretroviral therapy by two commercial genotyping methodologies. In individual subjects, the sequences in CSF samples differed significantly from those in plasma. In contrast, the viral sequences in CSF at the baseline did not differ from the sequences in CSF during treatment. Similarly, viral sequences in plasma did not vary over this interval. This study provides evidence that HIV-1 RNA in CSF and plasma arise from distinct compartments.

* Corresponding author. Mailing address: A3310 MCN, Division of Infectious Diseases, Vanderbilt University Medical Center, Nashville, TN 37232-2605. Phone: (615) 322-2035. Fax: (615) 343-6160. E-mail: yiwei.tang{at}vanderbilt.edu.

Journal of Clinical Microbiology, December 2000, p. 4637-4639, Vol. 38, No. 12
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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