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Journal of Clinical Microbiology, December 2000, p. 4646-4648, Vol. 38, No. 12
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Comparison of the Rodac Imprint Method to Selective Enrichment Broth for Recovery of Vancomycin-Resistant Enterococci and Drug-Resistant Enterobacteriaceae from Environmental Surfaces

Donna M. Hacek,1,* William E. Trick,2 Susan M. Collins,3 Gary A. Noskin,1,4,5 and Lance R. Peterson1,3,5

Northwestern Prevention EpiCenter,1 Clinical Microbiology Division, Department of Pathology,3 Department of Infection Control and Prevention,4 and Infectious Disease Division, Department of Medicine,5 Northwestern Memorial Hospital and Northwestern University Medical School, Chicago, Illinois, and Hospital Infections Program, Centers for Disease Control and Prevention, Atlanta, Georgia2

Received 3 August 2000/Returned for modification 16 September 2000/Accepted 28 September 2000

We compared the Rodac imprint technique to selective enrichment broth for detecting vancomycin-resistant enterococci (VRE) and multidrug-resistant Enterobacteriaceae (MDRE) on surfaces. Rodac plates contained tryptic soy agar with 5% sheep blood, vancomycin (6 µg/ml), ceftazidime (2 µg/ml), amphotericin B (2 µg/ml), and clindamycin (1 µg/ml). Two types of broth were used: brain heart infusion (BHI) and BHI plus vancomycin (6 µg/ml) and ceftazidime (2 µg/ml) (BHIVC). Of the 46 surfaces cultured for VRE, 12 (26%) were positive. Of the 12 VRE-positive surfaces, 11 (92%) grew from Rodac, 8 (67%) grew from BHIVC, and 7 (58%) grew from BHI. A larger study is needed for MDRE, as only 4 of 43 surfaces were MDRE positive. The Rodac imprint technique successfully recovered VRE from environmental surfaces.

* Corresponding author. Mailing address: Northwestern Prevention EpiCenter, Northwestern Memorial Hospital, Galter Carriage House, Suite 701, 251 E. Huron, Chicago, IL 60611. Phone: (312) 926-2885. Fax: (312) 926-4139. E-mail: dhacek{at}nmh.org.

Journal of Clinical Microbiology, December 2000, p. 4646-4648, Vol. 38, No. 12
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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