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Journal of Clinical Microbiology, December 2000, p. 4649-4652, Vol. 38, No. 12
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Characterization of Non-Type B Haemophilus influenzae Strains Isolated from Patients with Invasive Disease

Marina Cerquetti,1,* Marta Luisa Ciofi degli Atti,2 Giovanna Renna,1 Alberto Eugenio Tozzi,2 Maria Laura Garlaschi,3 Paola Mastrantonio,1 and The Hi Study Groupdagger

Laboratory of Bacteriology and Medical Mycology1 and Laboratory of Epidemiology and Biostatistics,2 Istituto Superiore di Sanità, 00161 Rome, and Azienda Ospedaliera Istituti Clinici di Perfezionamento, 20122 Milan,3 Italy

Received 5 May 2000/Returned for modification 6 July 2000/Accepted 10 September 2000

Forty-one non-type b Haemophilus influenzae isolates from cases of invasive disease were characterized. By PCR capsular genotyping, 33 nonencapsulated strains, 4 type f isolates, and 4 b- strains were identified. By pulsed-field gel electrophoresis, the nonencapsulated isolates exhibited great genetic heterogenicity, whereas the type f and the b- strains seemed to have a clonal spread. Occurrence of the hifA gene was found by PCR in 18% of the nonencapsulated, 50% of the b-, and all of the type f strains. Hemagglutinating fimbriae were generally expressed by nonencapsulated isolates when fimbrial gene hifA was present. Two nonencapsulated isolates not susceptible to ampicillin were detected; no strains were positive for beta -lactamase production.

* Corresponding author. Mailing address: Laboratorio di Batteriologia e Micologia Medica, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy. Phone: 3906 49902343. Fax: 3906 49387112. E-mail: mcerquet{at}iss.it.

dagger Members of The Hi Study Working Group: Istituto Superiore di Sanità, Laboratorio di Epidemiologia e Biostatistica, Patrizia Carbonari; Associazione Microbiologi Clinici Italiani (AMCLI), Pierluigi Nicoletti and Antonio Goglio; Regione Piemonte, Angela Ruggenini Moiraghi, Stefania Orecchia, Annalisa Castella, and Carla Zotti; Regione Lombardia, Alessandro Lizioli and Salvatore Pisani; Provincia Autonoma di Trento, Valter Carraro, Iole Caola, and Anna Calì; Regione Veneto, Giovanni Gallo; Regione Liguria, Pietro Crovari, Cristina Giordano, Pietro Tixi, and Marina Lemmi; Regione Toscana, Paolo Bonanni, Alessia Tomei, Patrizia Pecile, Emanuela Balocchini, and Licia Pecori; Regione Campania, Francesco Santonastasi, Loredana Cafaro, and Vittorio Pagano; Regione Puglia, Salvatore Barbuti and Maria Chironna.

Journal of Clinical Microbiology, December 2000, p. 4649-4652, Vol. 38, No. 12
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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