This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Blank, B. S. N.
Right arrow Articles by Lange, J. M. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Blank, B. S. N.
Right arrow Articles by Lange, J. M. A.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, February 2000, p. 563-569, Vol. 38, No. 2
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Value of Different Assays for Detection of Human Cytomegalovirus (HCMV) in Predicting the Development of HCMV Disease in Human Immunodeficiency Virus-Infected Patients

Brian S. N. Blank,1,2,* Pieter L. Meenhorst,2 Jan Willem Mulder,2 Gerrit Jan Weverling,1,3 Hein Putter,1 Wouter Pauw,4 Willemien C. van Dijk,4 Paul Smits,4 Sonja Lie-A-Ling,4 Peter Reiss,1 and Joep M. A. Lange1

National AIDS Therapy Evaluation Center, Department of Internal Medicine, Academic Medical Center, University of Amsterdam,1 Department of Internal Medicine2 and Department of Microbiology,4 Slotervaart Hospital, and Department of Clinical Epidemiology and Biostatistics, Academic Medical Center, University of Amsterdam,3 Amsterdam, The Netherlands

Received 19 January 1999/Returned for modification 26 April 1999/Accepted 1 November 1999

In the present prospective study, five blood tests for detection of human cytomegalovirus (HCMV), nucleic acid sequence-based amplification (NASBA) for detection of early (immediate-early antigen) and late (pp67) mRNA, PCR for detection of HCMV DNA (DNA PCR), culture, and pp65 antigenemia assay, and culture and DNA PCR of urine and throat swab specimens were compared for their abilities to predict the development of disease caused by HCMV (HCMV disease). Of 101 human immunodeficiency virus (HIV)-infected patients with <= 100 CD4+ lymphocytes per mm3, 25 patients developed HCMV disease. The pp65 antigenemia assay (sensitivity, 50%; specificity, 89%) and DNA PCR of blood (sensitivity, 69%; specificity, 75%) were most accurate in predicting the development of HCMV disease within the next 12 months. Both blood culture and late pp67 mRNA NASBA had high specificities (91 and 90%, respectively) but low sensitivities (25 and 13%, respectively). The sensitivities of urine culture, DNA PCR, throat swab specimen culture, DNA PCR, and NASBA of blood for detection of the immediate-early antigen were 73, 87, 53, 67, and 63%, respectively, and the specificities were 58, 46, 76, 60, and 72%, respectively. The positive predictive values of all tests however, were low and did not exceed 50%. In conclusion, virological screening by these qualitative assays for detection of HCMV is of limited value for prediction of the development of HCMV disease in HIV-infected patients.

* Corresponding author. Mailing address: National AIDS Evaluation Center, Department of Internal Medicine, Academic Medical Center, University of Amsterdam, Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands. Phone: 31 20 5664479. Fax: +31 20 6918821. E-mail: S.N.Blank{at}amc.uva.nl.

Journal of Clinical Microbiology, February 2000, p. 563-569, Vol. 38, No. 2
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Smits, P. H. M., Bakker, R., Jong, E., Mulder, J. W., Meenhorst, P. L., Kleter, B., van Doorn, L.-J., Quint, W. G. V. (2005). High Prevalence of Human Papillomavirus Infections in Urine Samples from Human Immunodeficiency Virus-Infected Men. J. Clin. Microbiol. 43: 5936-5939 [Abstract] [Full Text]  
  • Diaz-Mitoma, F., Leger, C., Miller, H., Giulivi, A., Frost, R., Shaw, L., Huebsch, L. (2003). Comparison of DNA Amplification, mRNA Amplification, and DNA Hybridization Techniques for Detection of Cytomegalovirus in Bone Marrow Transplant Recipients. J. Clin. Microbiol. 41: 5159-5166 [Abstract] [Full Text]  
  • Griscelli, F., Barrois, M., Chauvin, S., Lastere, S., Bellet, D., Bourhis, J.-H. (2001). Quantification of Human Cytomegalovirus DNA in Bone Marrow Transplant Recipients by Real-Time PCR. J. Clin. Microbiol. 39: 4362-4369 [Abstract] [Full Text]  
  • Bestetti, A., Pierotti, C., Terreni, M., Zappa, A., Vago, L., Lazzarin, A., Cinque, P. (2001). Comparison of Three Nucleic Acid Amplification Assays of Cerebrospinal Fluid for Diagnosis of Cytomegalovirus Encephalitis. J. Clin. Microbiol. 39: 1148-1151 [Abstract] [Full Text]  
  • Lipson, S. M., Della-Latta, P. (2000). Leukocyte Concentration in the Performance of the pp65 Antigenemia Assay. J. Clin. Microbiol. 38: 3137-3138 [Full Text]  
  • Zaia, J. A., Sissons, J.G. P., Riddell, S., Diamond, D. J., Wills, M.R., Carmichael, A.J., Weekes, M.P., Gandhi, M., La Rosa, C., Villacres, M., Lacey, S., Markel, S., Sun, J. (2000). Status of Cytomegalovirus Prevention and Treatment in 2000. ASH Education Book 2000: 339-355 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»