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Journal of Clinical Microbiology, February 2000, p. 625-629, Vol. 38, No. 2
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Pulsed-Field Gel Electrophoresis Analysis of Nasopharyngeal Flora in Children Attending a Day Care Center

Hisakazu Yano,1,2 Mitsuko Suetake,3 Akio Kuga,1 Kazuhiko Irinoda,1 Ryoichi Okamoto,1 Toshimitsu Kobayashi,2 and Matsuhisa Inoue1,*

Department of Microbiology, Kitasato University School of Medicine, Sagamihara, Kanagawa 228-8555,1 Department of Otolaryngology, Tohoku Rosai Hospital, Aoba-ku, Sendai 981-09113 and Department of Otolaryngology, Nagasaki University School of Medicine, Nagasaki 852-8501,2 Japan

Received 9 August 1999/Returned for modification 27 September 1999/Accepted 30 November 1999

To investigate how bacterial pathogens spread from child to child in a day care center, we monitored six children, two boys and four girls, born between August 1995 and November 1997, attending a day care center and analyzed nasopharyngeal samples from them using pulsed-field gel electrophoresis (PFGE). We obtained nasopharyngeal cultures from all of the affected children and almost all of the unaffected children between September 1998 and March 1999 after some children presented simultaneously with purulent rhinorrhea. Moreover, when a child was found to have acute otitis media, nasopharyngeal secretions from the child were independently cultured during treatment. During this period, 28 isolates of Moraxella catarrhalis, 13 of Streptococcus pneumoniae, and 4 of Haemophilus influenzae were recovered. PFGE gave 8 patterns for M. catarrhalis, 10 for S. pneumoniae, and 1 for H. influenzae. PFGE patterns demonstrated spread of M. catarrhalis between children. However, each occurrence of clusters of infection with M. catarrhalis lasted 2 to 6 weeks, with a change in PFGE pattern between occurrences of clusters. The M. catarrhalis strain infecting each child also changed. Similarly, the S. pneumoniae strain in each child also changed. In contrast, infection with H. influenzae persisted for about 3 months in an affected child.

* Corresponding author. Mailing address: Department of Microbiology, Kitasato University School of Medicine, Sagamihara, Kanagawa 228-8555, Japan. Phone: 81-42-778-9349. Fax: 81-42-778-9350. E-mail: matsu{at}kitasato-u.ac.jp.

Journal of Clinical Microbiology, February 2000, p. 625-629, Vol. 38, No. 2
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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