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Journal of Clinical Microbiology, February 2000, p. 651-655, Vol. 38, No. 2
0095-1137/00/$04.00+0
Comparison of Human Papillomavirus Detection and
Typing by Cycle Sequencing, Line Blotting, and Hybrid Capture
Suzanne D.
Vernon,*
Elizabeth R.
Unger, and
Deon
Williams
Centers for Disease Control and Prevention,
Atlanta, Georgia 30333
Received 23 August 1999/Returned for modification 11 October
1999/Accepted 11 November 1999
We compared the results of human papillomavirus (HPV)
detection and typing from 781 cervical samples assayed by three
methods: L1 consensus PCR followed by cycle sequencing, L1 consensus
PCR with biotinylated primers followed by hybridization to a line blot,
and Hybrid Capture assay. Both PCR assays used L1 consensus PCR with
primers MY09 and MY11. We evaluated the amplification efficiencies of
both PCR assays and also compared the specific HPV types detected by
each method. The samples positive by the Hybrid Capture assay were
compared to the specific types detected by the PCR-based assays. The
concordance between the two PCR assays in producing an HPV amplicon
visible by gel electrophoresis or in detecting any HPV type was
moderate: kappa values were 0.61 (95% confidence interval [CI] = 0.56 to 0.67) and 0.51 (95% CI = 0.46 to 0.58), respectively. The
McNemar test for correlated proportions indicated that biotinylated PCR
was less likely to produce a band (P = 0.001) and to
detect an HPV type (P = 0.001) than the other PCR
assay. In comparing the Hybrid Capture assay results with the HPV types
detected by the PCR-based assays, we found that positivity by the
Hybrid Capture assay for a number of samples may be due to
cross-hybridization with HPV types not included in the Hybrid Capture
assay probe cocktails.
*
Corresponding author. Mailing address: Centers for
Disease Control and Prevention, 1600 Clifton Rd., MSG18, Atlanta, GA
30333. Phone: (404) 639-2181. Fax: (404) 639-0049. E-mail:
sdv2{at}cdc.gov.
Journal of Clinical Microbiology, February 2000, p. 651-655, Vol. 38, No. 2
0095-1137/00/$04.00+0
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