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Journal of Clinical Microbiology, February 2000, p. 682-687, Vol. 38, No. 2
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Sequence Variation in the ftsZ Gene of Bartonella henselae Isolates and Clinical Samples

C. Ehrenborg,1 L. Wesslén,1 Å. Jakobson,2 G. Friman,1 and M. Holmberg1,*

Section of Infectious Diseases, Department of Medical Sciences,1 and Section of Pediatrics, Department of Women's and Children's Health,2 Uppsala University Hospital, Uppsala, Sweden

Received 19 July 1999/Returned for modification 25 August 1999/Accepted 3 November 1999

In a search for methods for subtyping of Bartonella henselae in clinical samples, we amplified and sequenced a 701-bp region in the 3' end of the ftsZ gene in 15 B. henselae isolates derived from cats and humans in the United States and Europe. The ftsZ sequence variants that were discovered were designated variants Bh ftsZ 1, 2, and 3 and were compared with 16S rRNA genotypes I and II of the same isolates. There was no ftsZ gene variation in the strains of 16S rRNA type I, all of which were Bh ftsZ 1. The type II strains constituted two groups, with nucleotide sequence variation in the ftsZ gene resulting in amino acid substitutions at three positions, one of which was shared by the two groups. One 16S rRNA type II isolate had an ftsZ gene sequence identical to those of the type I strains. Variants Bh ftsZ 1 and 2 were detected in tissue specimens from seven Swedish patients with diagnoses such as chronic multifocal osteomyelitis, cardiomyopathy, and lymphadenopathy. Patients with similar clinical entities displayed either Bh ftsZ variant. The etiological role of B. henselae in these patients was supported by positive Bartonella antibody titers and/or amplification and sequencing of a part of the B. henselae gltA gene. B. henselae ftsZ gene sequence variation may be useful in providing knowledge about the epidemiology of various B. henselae strains in clinical samples, especially when isolation attempts have failed. This report also describes manifestations of atypical Bartonella infections in Sweden.

* Corresponding author. Mailing address: Section of Infectious Diseases, Uppsala University Hospital, 751 85 Uppsala, Sweden. Phone: 46-18-66 56 62. Fax: 46-18-665650. E-mail: martin.holmberg{at}infektion.uu.se.

Journal of Clinical Microbiology, February 2000, p. 682-687, Vol. 38, No. 2
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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