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Journal of Clinical Microbiology, February 2000, p. 752-754, Vol. 38, No. 2
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Correlation of Oxacillin MIC with mecA Gene Carriage in Coagulase-Negative Staphylococci

Zafar Hussain,1,2,* Luba Stoakes,1 Viki Massey,1 Deb Diagre,1 Viivi Fitzgerald,1 Sameer El Sayed,1,2 and Robert Lannigan1,2

London Health Sciences Centre1 and Department of Microbiology and Immunology, The University of Western Ontario,2 London, Ontario, Canada

Received 7 July 1999/Returned for modification 26 August 1999/Accepted 12 November 1999

The National Committee for Clinical Laboratory Standards has recently changed the oxacillin breakpoint from >= 4 mg/liter to >= 0.5 mg/liter to detect methicillin-resistant coagulase-negative staphylococci (CoNS) because the previous breakpoint lacked sensitivity. To determine the correlation between the new oxacillin breakpoint and the presence of the mecA gene, 493 CoNS of 11 species were tested. The presence of the mecA gene was determined by PCR, and oxacillin susceptibility was determined by the agar dilution method with Mueller-Hinton agar containing 2% NaCl and oxacillin (0.125 to 4.0 mg/liter). The new breakpoint correctly classified all CoNS strains with mecA as methicillin resistant and strains of Staphylococcus epidermidis, S. haemolyticus, and S. hominis without mecA as methicillin susceptible. The breakpoint of >= 0.5 mg/liter was not specific for S. cohnii, S. lugdunensis, S. saprophyticus, S. warneri, and S. xylosus, in that it categorized 70 of 74 strains of these species without mecA (94.6%) as methicillin resistant. The results of this study indicate that the new oxacillin breakpoint accurately identifies strains of CoNS with mecA but is not specific for strains of certain species of CoNS without mecA.

* Corresponding author. Mailing address: Department of Clinical Microbiology and Infection Control, Westminster Tower, London Health Sciences Centre, Box 5010, London, Ontario, Canada N6A 4G5. Phone: (519) 685-8149. Fax: (519) 685-8203. E-mail: Zafar.hussain{at}lhsc.on.ca.

Journal of Clinical Microbiology, February 2000, p. 752-754, Vol. 38, No. 2
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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