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Journal of Clinical Microbiology, February 2000, p. 763-767, Vol. 38, No. 2
Department of Bacteriology and Immunology,
Haartman Institute and HD Laboratories, University and University
Central Hospital of Helsinki,1 and
Department of Obstetrics and Gynecology, Peijas Hospital,
Vantaa,2 Finland
Received 6 July 1999/Returned for modification 15 October
1999/Accepted 13 November 1999
Trichomonas vaginalis is a globally common sexually
transmitted human parasite. Many strains of T. vaginalis from around the world have been described to be
resistant to the current drug of choice, metronidazole. However, only a
few cases of metronidazole resistance have been reported from Europe.
The resistant strains cause prolonged infections which are difficult to
treat. T. vaginalis infection also increases the risk for
human immunodeficiency virus transmission. We present a practical
method for determining the resistance of T. vaginalis to 5-nitroimidazoles. The suggested method was
developed by determining the MICs and minimal lethal concentrations (MLCs) of metronidazole and ornidazole for
T. vaginalis under various aerobic and anaerobic
conditions. Using this assay we have found the first three
metronidazole-resistant strains from Finland, although the origin
of at least one of the strains seems to be Russia. Analysis of the
patient-derived and previously characterized isolates
showed that metronidazole-resistant strains were also resistant to
ornidazole, and MLCs for all strains tested correlated well with the
MICs. The suggested MICs of metronidazole for differentiation
of sensitive and resistant isolates are >75 µg/ml in an
aerobic 24-h assay and >15 µg/ml in an anaerobic 48-h assay.
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Resistance of Trichomonas vaginalis to Metronidazole:
Report of the First Three Cases from Finland and Optimization of
In Vitro Susceptibility Testing under Various Oxygen
Concentrations
*
Corresponding author. Mailing address: Haartman
Institute, P.O. Box 21, FIN-00029 University of Helsinki, Finland.
Phone: 358-9-1912 6229. Fax: 358-9-1912 6382. E-mail:
Taru.Meri{at}helsinki.fi.
Journal of Clinical Microbiology, February 2000, p. 763-767, Vol. 38, No. 2
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
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