This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Weinberg, A.
Right arrow Articles by Zamora, M. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Weinberg, A.
Right arrow Articles by Zamora, M. R.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, February 2000, p. 768-772, Vol. 38, No. 2
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Comparison of PCR, Antigenemia Assay, and Rapid Blood Culture for Detection and Prevention of Cytomegalovirus Disease after Lung Transplantation

Adriana Weinberg,* Tony N. Hodges, Shaobing Li, Guanyung Cai, and Martin R. Zamora

Diagnostic Virology Laboratory of the Department of Pediatrics and Respiratory Medicine, University of Colorado Health Sciences Center, Denver, Colorado

Received 25 August 1999/Returned for modification 22 October 1999/Accepted 11 November 1999

The goal of this study was to evaluate serial cytomegalovirus (CMV) blood culture, antigenemia testing, and qualitative and quantitative plasma CMV PCR for their ability to predict CMV disease and thus to direct preemptive therapy after lung transplantation. Forty-one patients provided 414 samples for blood culture, 290 samples for antigenemia testing, and 432 samples for PCR. Seven patients developed 11 episodes of CMV disease. CMV PCR had sensitivity, specificity, and positive predictive and negative predictive values of 79, 99, 84, and 99%, respectively, compared with 48, 99, 85, and 98%, respectively, for antigenemia testing, and 8, 100, 100, and 97%, respectively, for culture. Only quantitative CMV PCR correlated with disease stage: asymptomatic patients had a mean of 1,500 CMV DNA copies/ml, whereas patients who developed CMV disease had 5,087 copies/ml 12 to 4 weeks before symptoms and 32,000 copies/ml at diagnosis. Furthermore, CMV PCR-measured DNA increased 5- to 10-fold immediately preceding symptoms. PCR and antigenemia test values decreased with anti-CMV therapy. CMV DNA (as detected by PCR), but not antigenemia, persisted in patients who later developed recurrent CMV disease. The data indicate that lung transplant recipients will benefit from monitoring of CMV disease by plasma CMV PCR.

* Corresponding author. Mailing address: University of Colorado Health Sciences Center, Campus box C227, 4200 East Ninth Ave., Denver, CO 80262. Phone: (303) 315-4624. Fax: (303) 315-6955. E-mail: adriana.weinberg{at}uchsc.edu.

Journal of Clinical Microbiology, February 2000, p. 768-772, Vol. 38, No. 2
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • D'Journo, X. B., Michelet, P., Papazian, L., Reynaud-Gaubert, M., Doddoli, C., Giudicelli, R., Fuentes, P. A., Thomas, P. A. (2008). Airway colonisation and postoperative pulmonary complications after neoadjuvant therapy for oesophageal cancer. Eur. J. Cardiothorac. Surg. 33: 444-450 [Abstract] [Full Text]  
  • Herrmann, B., Cavaglia Larsson, V., Rubin, C.-J., Sund, F., Eriksson, B.-M., Arvidson, J., Yun, Z., Bondeson, K., Blomberg, J. (2004). Comparison of a Duplex Quantitative Real-Time PCR Assay and the COBAS Amplicor CMV Monitor Test for Detection of Cytomegalovirus. J. Clin. Microbiol. 42: 1909-1914 [Abstract] [Full Text]  
  • Mengelle, C., Sandres-Saune, K., Pasquier, C., Rostaing, L., Mansuy, J.-M., Marty, M., Da Silva, I., Attal, M., Massip, P., Izopet, J. (2003). Automated Extraction and Quantification of Human Cytomegalovirus DNA in Whole Blood by Real-Time PCR Assay. J. Clin. Microbiol. 41: 3840-3845 [Abstract] [Full Text]  
  • Stocher, M., Berg, J. (2002). Normalized Quantification of Human Cytomegalovirus DNA by Competitive Real-Time PCR on the LightCycler Instrument. J. Clin. Microbiol. 40: 4547-4553 [Abstract] [Full Text]  
  • Weinberg, A., Schissel, D., Giller, R. (2002). Molecular Methods for Cytomegalovirus Surveillance in Bone Marrow Transplant Recipients. J. Clin. Microbiol. 40: 4203-4206 [Abstract] [Full Text]  
  • Chakinala, M. M., Kollef, M. H., Trulock, E. P. (2002). Critical Care Aspects of Lung Transplant Patients. J Intensive Care Med 17: 8-33 [Abstract]  
  • Solano, C., Munoz, I., Gutierrez, A., Farga, A., Prosper, F., Garcia-Conde, J., Navarro, D., Gimeno, C. (2001). Qualitative Plasma PCR Assay (AMPLICOR CMV Test) versus pp65 Antigenemia Assay for Monitoring Cytomegalovirus Viremia and Guiding Preemptive Ganciclovir Therapy in Allogeneic Stem Cell Transplantation. J. Clin. Microbiol. 39: 3938-3941 [Abstract] [Full Text]  
  • Lurain, N. S., Weinberg, A., Crumpacker, C. S., Chou, S. (2001). Sequencing of Cytomegalovirus UL97 Gene for Genotypic Antiviral Resistance Testing. Antimicrob. Agents Chemother. 45: 2775-2780 [Abstract] [Full Text]  
  • Caliendo, A. M., Schuurman, R., Yen-Lieberman, B., Spector, S. A., Andersen, J., Manjiry, R., Crumpacker, C., Lurain, N. S., Erice, A. (2001). Comparison of Quantitative and Qualitative PCR Assays for Cytomegalovirus DNA in Plasma. J. Clin. Microbiol. 39: 1334-1338 [Abstract] [Full Text]  
  • Witt, D. J., Kemper, M., Stead, A., Sillekens, P., Ginocchio, C. C., Espy, M. J., Paya, C. V., Smith, T. F., Roeles, F., Caliendo, A. M. (2000). Analytical Performance and Clinical Utility of a Nucleic Acid Sequence-Based Amplification Assay for Detection of Cytomegalovirus Infection. J. Clin. Microbiol. 38: 3994-3999 [Abstract] [Full Text]  
  • Schaade, L., Kockelkorn, P., Ritter, K., Kleines, M. (2000). Detection of Cytomegalovirus DNA in Human Specimens by LightCycler PCR. J. Clin. Microbiol. 38: 4006-4009 [Abstract] [Full Text]  
  • Bai, X., Rogers, B. B., Harkins, P. C., Sommerauer, J., Squires, R., Rotondo, K., Quan, A., Dawson, D. B., Scheuermann, R. H. (2000). Predictive Value of Quantitative PCR-Based Viral Burden Analysis for Eight Human Herpesviruses in Pediatric Solid Organ Transplant Patients. J. Mol. Diagn. 2: 191-201 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»