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Journal of Clinical Microbiology, February 2000, p. 789-794, Vol. 38, No. 2
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Application of 16S rRNA Gene Sequencing To Identify
Bordetella hinzii as the Causative Agent of Fatal
Septicemia
M. M.
Kattar,1
J. F.
Chavez,2
A. P.
Limaye,1,3
S. L.
Rassoulian-Barrett,1
S. L.
Yarfitz,4
L. C.
Carlson,1
Y.
Houze,1
S.
Swanzy,1
B. L.
Wood,1 and
B. T.
Cookson1,5,*
Departments of Laboratory
Medicine,1 Infectious
Diseases,3 and
Microbiology,5 and Health
Sciences Library and Department of Medical Education, Division of
Bioinformatics,4 University of Washington,
Seattle, Washington, and Department of Infectious
Diseases, Washington Veterans Affairs Medical Center, Washington,
D.C.2
Received 24 August 1999/Returned for modification 25 October
1999/Accepted 23 November 1999
We report on the first case of fatal septicemia caused by
Bordetella hinzii. The causative organism exhibited a
biochemical profile identical to that of Bordetella avium
with three commercial identification systems (API 20E, API 20 NE, and
Vitek GNI+ card). However, its cellular fatty acid profile was not
typical for either B. avium or previously reported strains
of B. hinzii. Presumptive identification of the patient's
isolate was accomplished by traditional biochemical testing, and
definitive identification was achieved by 16S rRNA gene sequence
analysis. Phenotypic features useful in distinguishing B. hinzii from B. avium were production of alkali from
malonate and resistance to several antimicrobial agents.
*
Corresponding author. Mailing address: Departments of
Laboratory Medicine and Microbiology, University of Washington, 1959 NE
Pacific St., NW 120, Box 357110, Seattle, WA 98195. Phone: (206)
598-6131. Fax: (206) 598-6189. E-mail:
cookson{at}u.washington.edu.
Journal of Clinical Microbiology, February 2000, p. 789-794, Vol. 38, No. 2
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
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