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Journal of Clinical Microbiology, February 2000, p. 789-794, Vol. 38, No. 2
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Application of 16S rRNA Gene Sequencing To Identify Bordetella hinzii as the Causative Agent of Fatal Septicemia

M. M. Kattar,1 J. F. Chavez,2 A. P. Limaye,1,3 S. L. Rassoulian-Barrett,1 S. L. Yarfitz,4 L. C. Carlson,1 Y. Houze,1 S. Swanzy,1 B. L. Wood,1 and B. T. Cookson1,5,*

Departments of Laboratory Medicine,1 Infectious Diseases,3 and Microbiology,5 and Health Sciences Library and Department of Medical Education, Division of Bioinformatics,4 University of Washington, Seattle, Washington, and Department of Infectious Diseases, Washington Veterans Affairs Medical Center, Washington, D.C.2

Received 24 August 1999/Returned for modification 25 October 1999/Accepted 23 November 1999

We report on the first case of fatal septicemia caused by Bordetella hinzii. The causative organism exhibited a biochemical profile identical to that of Bordetella avium with three commercial identification systems (API 20E, API 20 NE, and Vitek GNI+ card). However, its cellular fatty acid profile was not typical for either B. avium or previously reported strains of B. hinzii. Presumptive identification of the patient's isolate was accomplished by traditional biochemical testing, and definitive identification was achieved by 16S rRNA gene sequence analysis. Phenotypic features useful in distinguishing B. hinzii from B. avium were production of alkali from malonate and resistance to several antimicrobial agents.

* Corresponding author. Mailing address: Departments of Laboratory Medicine and Microbiology, University of Washington, 1959 NE Pacific St., NW 120, Box 357110, Seattle, WA 98195. Phone: (206) 598-6131. Fax: (206) 598-6189. E-mail: cookson{at}u.washington.edu.

Journal of Clinical Microbiology, February 2000, p. 789-794, Vol. 38, No. 2
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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