This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kempf, V. A. J.
Right arrow Articles by Autenrieth, I. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kempf, V. A. J.
Right arrow Articles by Autenrieth, I. B.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, February 2000, p. 830-838, Vol. 38, No. 2
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Fluorescent In Situ Hybridization Allows Rapid Identification of Microorganisms in Blood Cultures

Volkhard A. J. Kempf, Karlheinz Trebesius, and Ingo B. Autenrieth*

Max von Pettenkofer-Institut für Hygiene und Medizinische Mikrobiologie, Ludwig Maximilians Universität München, D-80336 Munich, Germany

Received 30 July 1999/Returned for modification 20 September 1999/Accepted 20 October 1999

Using fluorescent in situ hybridization (FISH) with rRNA-targeted fluorescently labelled oligonucleotide probes, pathogens were rapidly detected and identified in positive blood culture bottles without cultivation and biotyping. In this study, 115 blood cultures with a positive growth index as determined by a continuous-reading automated blood culture system were examined by both conventional laboratory methods and FISH. For this purpose, oligonucleotide probes that allowed identification of approximately 95% of those pathogens typically associated with bacteremia were produced. The sensitivity and specificity of these probes were 100%. From all 115 blood cultures, microorganisms were grown after 1 day and identification to the family, genus, or species level was achieved after 1 to 3 days while 111 samples (96.5%) were similarly identified by FISH within 2.5 h. Staphylococci were identified in 62 of 62 samples, streptococci and enterococci were identified in 19 of 20 samples, gram-negative rods were identified in 28 of 30 samples, and fungi were identified in two of two samples. Thus, FISH is an appropriate method for identification of pathogens grown in blood cultures from septicemic patients.

* Corresponding author. Mailing address: Max von Pettenkofer-Institut, Ludwig Maximilians Universität, Pettenkoferstr. 9a, D-80336 Munich, Germany. Phone: 0049-89-51605280. Fax: 0049-89-51605223. E-mail: Autenrieth{at}m3401.mpk.med.uni-muenchen.de.

Journal of Clinical Microbiology, February 2000, p. 830-838, Vol. 38, No. 2
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Tavares, A., Inacio, J., Melo-Cristino, J., Couto, I. (2008). Use of Fluorescence In Situ Hybridization for Rapid Identification of Staphylococci in Blood Culture Samples Collected in a Portuguese Hospital. J. Clin. Microbiol. 46: 3097-3100 [Abstract] [Full Text]  
  • Narisawa, N., Haruta, S., Arai, H., Ishii, M., Igarashi, Y. (2008). Coexistence of Antibiotic-Producing and Antibiotic-Sensitive Bacteria in Biofilms Is Mediated by Resistant Bacteria. Appl. Environ. Microbiol. 74: 3887-3894 [Abstract] [Full Text]  
  • Poppert, S., Haas, M., Yildiz, T., Alter, T., Bartel, E., Fricke, U., Essig, A. (2008). Identification of Thermotolerant Campylobacter Species by Fluorescence In Situ Hybridization. J. Clin. Microbiol. 46: 2133-2136 [Abstract] [Full Text]  
  • Werner, G., Bartel, M., Wellinghausen, N., Essig, A., Klare, I., Witte, W., Poppert, S. (2007). Detection of Mutations Conferring Resistance to Linezolid in Enterococcus spp. by Fluorescence In Situ Hybridization. J. Clin. Microbiol. 45: 3421-3423 [Abstract] [Full Text]  
  • Wellinghausen, N., Bartel, M., Essig, A., Poppert, S. (2007). Rapid Identification of Clinically Relevant Enterococcus Species by Fluorescence In Situ Hybridization. J. Clin. Microbiol. 45: 3424-3426 [Abstract] [Full Text]  
  • Sogaard, M., Hansen, D. S., Fiandaca, M. J., Stender, H., Schonheyder, H. C. (2007). Peptide nucleic acid fluorescence in situ hybridization for rapid detection of Klebsiella pneumoniae from positive blood cultures. J Med Microbiol 56: 914-917 [Abstract] [Full Text]  
  • Tang, Y.-W., Kilic, A., Yang, Q., McAllister, S. K., Li, H., Miller, R. S., McCormac, M., Tracy, K. D., Stratton, C. W., Han, J., Limbago, B. (2007). StaphPlex System for Rapid and Simultaneous Identification of Antibiotic Resistance Determinants and Panton-Valentine Leukocidin Detection of Staphylococci from Positive Blood Cultures. J. Clin. Microbiol. 45: 1867-1873 [Abstract] [Full Text]  
  • Ng, S. Y., Kwang, L. L., Tan, T. Y. (2007). Identification of Gram-negative bacilli directly from positive blood culture vials. J Med Microbiol 56: 475-479 [Abstract] [Full Text]  
  • Peters, R. P. H., van Agtmael, M. A., Simoons-Smit, A. M., Danner, S. A., Vandenbroucke-Grauls, C. M. J. E., Savelkoul, P. H. M. (2006). Rapid identification of pathogens in blood cultures with a modified fluorescence in situ hybridization assay.. J. Clin. Microbiol. 44: 4186-4188 [Abstract] [Full Text]  
  • Hall-Stoodley, L., Hu, F. Z., Gieseke, A., Nistico, L., Nguyen, D., Hayes, J., Forbes, M., Greenberg, D. P., Dice, B., Burrows, A., Wackym, P. A., Stoodley, P., Post, J. C., Ehrlich, G. D., Kerschner, J. E. (2006). Direct detection of bacterial biofilms on the middle-ear mucosa of children with chronic otitis media.. JAMA 296: 202-211 [Abstract] [Full Text]  
  • Cleven, B. E. E., Palka-Santini, M., Gielen, J., Meembor, S., Kronke, M., Krut, O. (2006). Identification and characterization of bacterial pathogens causing bloodstream infections by DNA microarray.. J. Clin. Microbiol. 44: 2389-2397 [Abstract] [Full Text]  
  • Wellinghausen, N., Nockler, K., Sigge, A., Bartel, M., Essig, A., Poppert, S. (2006). Rapid Detection of Brucella spp. in Blood Cultures by Fluorescence In Situ Hybridization.. J. Clin. Microbiol. 44: 1828-1830 [Abstract] [Full Text]  
  • Scupham, A. J, Presley, L. L., Wei, B., Bent, E., Griffith, N., McPherson, M., Zhu, F., Oluwadara, O., Rao, N., Braun, J., Borneman, J. (2006). Abundant and Diverse Fungal Microbiota in the Murine Intestine. Appl. Environ. Microbiol. 72: 793-801 [Abstract] [Full Text]  
  • Peters, R. P. H., Savelkoul, P. H. M., Simoons-Smit, A. M., Danner, S. A., Vandenbroucke-Grauls, C. M. J. E., van Agtmael, M. A. (2006). Faster Identification of Pathogens in Positive Blood Cultures by Fluorescence In Situ Hybridization in Routine Practice. J. Clin. Microbiol. 44: 119-123 [Abstract] [Full Text]  
  • Brown, D. F. J., Edwards, D. I., Hawkey, P. M., Morrison, D., Ridgway, G. L., Towner, K. J., Wren, M. W. D., on behalf of the Joint Working Party of the Britis, (2005). Guidelines for the laboratory diagnosis and susceptibility testing of methicillin-resistant Staphylococcus aureus (MRSA). J Antimicrob Chemother 56: 1000-1018 [Abstract] [Full Text]  
  • Hartmann, H., Stender, H., Schafer, A., Autenrieth, I. B., Kempf, V. A. J. (2005). Rapid Identification of Staphylococcus aureus in Blood Cultures by a Combination of Fluorescence In Situ Hybridization Using Peptide Nucleic Acid Probes and Flow Cytometry. J. Clin. Microbiol. 43: 4855-4857 [Abstract] [Full Text]  
  • Mandle, T., Einsele, H., Schaller, M., Neumann, D., Vogel, W., Autenrieth, I. B., Kempf, V. A. J. (2005). Infection of human CD34+ progenitor cells with Bartonella henselae results in intraerythrocytic presence of B henselae. Blood 106: 1215-1222 [Abstract] [Full Text]  
  • Wellinghausen, N., Kothe, J., Wirths, B., Sigge, A., Poppert, S. (2005). Superiority of Molecular Techniques for Identification of Gram-Negative, Oxidase-Positive Rods, Including Morphologically Nontypical Pseudomonas aeruginosa, from Patients with Cystic Fibrosis. J. Clin. Microbiol. 43: 4070-4075 [Abstract] [Full Text]  
  • Poppert, S., Essig, A., Stoehr, B., Steingruber, A., Wirths, B., Juretschko, S., Reischl, U., Wellinghausen, N. (2005). Rapid Diagnosis of Bacterial Meningitis by Real-Time PCR and Fluorescence In Situ Hybridization. J. Clin. Microbiol. 43: 3390-3397 [Abstract] [Full Text]  
  • Wilson, D. A., Joyce, M. J., Hall, L. S., Reller, L. B., Roberts, G. D., Hall, G. S., Alexander, B. D., Procop, G. W. (2005). Multicenter Evaluation of a Candida albicans Peptide Nucleic Acid Fluorescent In Situ Hybridization Probe for Characterization of Yeast Isolates from Blood Cultures. J. Clin. Microbiol. 43: 2909-2912 [Abstract] [Full Text]  
  • Lindholm, L., Sarkkinen, H. (2004). Direct Identification of Gram-Positive Cocci from Routine Blood Cultures by Using AccuProbe Tests. J. Clin. Microbiol. 42: 5609-5613 [Abstract] [Full Text]  
  • Sakai, H., Procop, G. W., Kobayashi, N., Togawa, D., Wilson, D. A., Borden, L., Krebs, V., Bauer, T. W. (2004). Simultaneous Detection of Staphylococcus aureus and Coagulase-Negative Staphylococci in Positive Blood Cultures by Real-Time PCR with Two Fluorescence Resonance Energy Transfer Probe Sets. J. Clin. Microbiol. 42: 5739-5744 [Abstract] [Full Text]  
  • Wellinghausen, N., Wirths, B., Essig, A., Wassill, L. (2004). Evaluation of the Hyplex BloodScreen Multiplex PCR-Enzyme-Linked Immunosorbent Assay System for Direct Identification of Gram-Positive Cocci and Gram-Negative Bacilli from Positive Blood Cultures. J. Clin. Microbiol. 42: 3147-3152 [Abstract] [Full Text]  
  • Iwen, P. C., Freifeld, A. G., Bruening, T. A., Hinrichs, S. H. (2004). Use of a Panfungal PCR Assay for Detection of Fungal Pathogens in a Commercial Blood Culture System. J. Clin. Microbiol. 42: 2292-2293 [Abstract] [Full Text]  
  • Marlowe, E. M., Hogan, J. J., Hindler, J. F., Andruszkiewicz, I., Gordon, P., Bruckner, D. A. (2003). Application of an rRNA Probe Matrix for Rapid Identification of Bacteria and Fungi from Routine Blood Cultures. J. Clin. Microbiol. 41: 5127-5133 [Abstract] [Full Text]  
  • Levi, K., Towner, K. J. (2003). Detection of Methicillin-Resistant Staphylococcus aureus (MRSA) in Blood with the EVIGENE MRSA Detection Kit. J. Clin. Microbiol. 41: 3890-3892 [Abstract] [Full Text]  
  • Bertaux, J., Schmid, M., Prevost-Boure, N. C., Churin, J. L., Hartmann, A., Garbaye, J., Frey-Klett, P. (2003). In Situ Identification of Intracellular Bacteria Related to Paenibacillus spp. in the Mycelium of the Ectomycorrhizal Fungus Laccaria bicolor S238N. Appl. Environ. Microbiol. 69: 4243-4248 [Abstract] [Full Text]  
  • Inacio, J., Behrens, S., Fuchs, B. M., Fonseca, A., Spencer-Martins, I., Amann, R. (2003). In Situ Accessibility of Saccharomyces cerevisiae 26S rRNA to Cy3-Labeled Oligonucleotide Probes Comprising the D1 and D2 Domains. Appl. Environ. Microbiol. 69: 2899-2905 [Abstract] [Full Text]  
  • Artz, L. A., Kempf, V. A. J., Autenrieth, I. B. (2003). Rapid Screening for Streptococcus agalactiae in Vaginal Specimens of Pregnant Women by Fluorescent In Situ Hybridization. J. Clin. Microbiol. 41: 2170-2173 [Abstract] [Full Text]  
  • Marlowe, E. M., Gibson, L., Hogan, J., Kaplan, S., Bruckner, D. A. (2003). Conventional and Molecular Methods for Verification of Results Obtained with BacT/Alert Nonvent Blood Culture Bottles. J. Clin. Microbiol. 41: 1266-1269 [Abstract] [Full Text]  
  • Oliveira, K., Brecher, S. M., Durbin, A., Shapiro, D. S., Schwartz, D. R., De Girolami, P. C., Dakos, J., Procop, G. W., Wilson, D., Hanna, C. S., Haase, G., Peltroche-Llacsahuanga, H., Chapin, K. C., Musgnug, M. C., Levi, M. H., Shoemaker, C., Stender, H. (2003). Direct Identification of Staphylococcus aureus from Positive Blood Culture Bottles. J. Clin. Microbiol. 41: 889-891 [Abstract] [Full Text]  
  • Poppert, S., Essig, A., Marre, R., Wagner, M., Horn, M. (2002). Detection and Differentiation of Chlamydiae by Fluorescence In Situ Hybridization. Appl. Environ. Microbiol. 68: 4081-4089 [Abstract] [Full Text]  
  • Rigby, S., Procop, G. W., Haase, G., Wilson, D., Hall, G., Kurtzman, C., Oliveira, K., Von Oy, S., Hyldig-Nielsen, J. J., Coull, J., Stender, H. (2002). Fluorescence In Situ Hybridization with Peptide Nucleic Acid Probes for Rapid Identification of Candida albicans Directly from Blood Culture Bottles. J. Clin. Microbiol. 40: 2182-2186 [Abstract] [Full Text]  
  • Oliveira, K., Procop, G. W., Wilson, D., Coull, J., Stender, H. (2002). Rapid Identification of Staphylococcus aureus Directly from Blood Cultures by Fluorescence In Situ Hybridization with Peptide Nucleic Acid Probes. J. Clin. Microbiol. 40: 247-251 [Abstract] [Full Text]  
  • Mbuthia, P. G., Christensen, H., Boye, M., Petersen, K. M. D., Bisgaard, M., Nyaga, P. N., Olsen, J. E. (2001). Specific Detection of Pasteurella multocida in Chickens with Fowl Cholera and in Pig Lung Tissues Using Fluorescent rRNA In Situ Hybridization. J. Clin. Microbiol. 39: 2627-2633 [Abstract] [Full Text]  
  • Rüssmann, H., Kempf, V. A. J., Koletzko, S., Heesemann, J., Autenrieth, I. B. (2001). Comparison of Fluorescent In Situ Hybridization and Conventional Culturing for Detection of Helicobacter pylori in Gastric Biopsy Specimens. J. Clin. Microbiol. 39: 304-308 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»