Simultaneous Approach for Nonculture PCR-Based Identification and Serogroup Prediction of Neisseria meningitidis

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Journal of Clinical Microbiology, February 2000, p. 855-857, Vol. 38, No. 2
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Simultaneous Approach for Nonculture PCR-Based Identification and Serogroup Prediction of Neisseria meningitidis

Muhamed-Kheir Taha^*

Unité des Neisseria and Centre National de Référence des Meningocoques, Institut Pasteur, 75724 Paris cedex 15, France

Received 27 May 1999/Returned for modification 12 August 1999/Accepted 4 November 1999

A nonculture PCR-based method to characterize Neisseria meningitidis was used to test 225 clinical specimens. PCR correctlyidentified and predicted the serogroups of N. meningitidis ofculture-proven meningococcal diseases and confirmed this diagnosisin 35% of suspected samples. This approach could be useful whenculture fails to isolate N. meningitidis.

^* Mailing address: Unité des Neisseria and Centre National de Référence des Méningocoques, Institut Pasteur, 28 rue du DrRoux, 75724 Paris cedex 15, France. Phone: 33 1 45 68 84 38. Fax:33 1 45 68 83 38. E-mail: mktaha{at}pasteur.fr.

Journal of Clinical Microbiology, February 2000, p. 855-857, Vol. 38, No. 2
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

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