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Journal of Clinical Microbiology, February 2000, p. 858-860, Vol. 38, No. 2
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Comparison of Blood Collected in Acid-Citrate-Dextrose and EDTA for Use in Human Immunodeficiency Virus Peripheral Blood Mononuclear Cell Cultures

Susan A. Fiscus,1,* Hrishikesh Chakraborty,2 Robin Shepard,1 and Melissa Goodman1

Department of Microbiology & Immunology1 and Department of Biostatistics,2 University of North Carolina at Chapel Hill, Chapel Hill, North Carolina

Received 23 June 1999/Returned for modification 26 August 1999/Accepted 27 September 1999

Paired blood samples collected in acid-citrate-dextrose and EDTA were compared for human immunodeficiency virus (HIV) infectivity on the day of collection or after 1 day of storage at room temperature. No significant differences between the anticoagulants were observed. Culture positivity was significantly associated with HIV RNA viral loads for both anticoagulants.

* Corresponding author. Mailing address: Department of Microbiology & Immunology, University of North Carolina at Chapel Hill, CB# 7140, Chapel Hill, NC 27599-7140. Phone: (919) 966-9872. Fax: (919) 966-9873. E-mail: fiscussa{at}med.unc.edu.

Journal of Clinical Microbiology, February 2000, p. 858-860, Vol. 38, No. 2
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Jennings, C., Bremer, J. W., Brambilla, D. J. (2000). Investigation of Effects of Acid Citrate Dextrose and EDTA on Ability To Quantitatively Culture Human Immunodeficiency Virus. J. Clin. Microbiol. 38: 3522-3522 [Full Text]  


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