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Journal of Clinical Microbiology, February 2000, p. 861-862, Vol. 38, No. 2
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Effect of a Mycoplasma hominis-Like Mycoplasma on the Infection of HEp-2 Cells by the TW-183 Strain of Chlamydia pneumoniae

Elias A. Castilla1,2 and Robert M. Wadowsky1,2,3,*

Department of Pathology, School of Medicine,1 and Department of Infectious Diseases and Microbiology, Graduate School of Public Health,3 University of Pittsburgh, Pittsburgh, Pennsylvania 15261, and Children's Hospital of Pittsburgh, Pittsburgh, Pennsylvania 15213-25832

Received 14 June 1999/Returned for modification 22 October 1999/Accepted 11 November 1999

We isolated a Mycoplasma hominis-like mycoplasma from a stock culture of Chlamydia pneumoniae TW-183 obtained from the American Type Culture Collection and eradicated the contaminant by treating the stock suspension with a nonionic detergent, Igepal CA-630. The M. hominis-like mycoplasma neither inhibits nor enhances the infectivity of C. pneumoniae for HEp-2 cells.


* Corresponding author. Mailing address: Department of Pathology, Children's Hospital of Pittsburgh, 3705 Fifth Ave., Pittsburgh, PA 15213-2583. Phone: (412) 692-5314. Fax: (412) 692-6550. E-mail: wadowsr{at}chplink.chp.edu.


Journal of Clinical Microbiology, February 2000, p. 861-862, Vol. 38, No. 2
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.



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