Presence of Type III Secretion Genes in Burkholderia pseudomallei Correlates with Ara- Phenotypes

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Journal of Clinical Microbiology, February 2000, p. 883-885, Vol. 38, No. 2
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Presence of Type III Secretion Genes in Burkholderia pseudomallei Correlates with Ara Phenotypes

Craig Winstanley^* and C. Anthony Hart

Department of Medical Microbiology and Genito-Urinary Medicine, University of Liverpool, Liverpool L69 3GA, United Kingdom

Received 29 July 1999/Returned for modification 17 September 1999/Accepted 1 November 1999

Dot blot hybridization and PCR amplification of 14 Ara⁺ and 8 Ara Burkholderia pseudomallei strains showed that type III secretion(TTS) genes were present in all the Ara strains but absent from all but one of the Ara⁺ strains. The link between TTS genes and an Ara phenotype suggests a role for TTS invirulence.

^* Corresponding author. Department of Medical Microbiology and Genito-Urinary Medicine, University of Liverpool, P.O. Box 147,Liverpool L69 3BX, United Kingdom. Phone: 44 (0)151 706 2000,ext. 4388. Fax: 44 (0)151 706 5805. E-mail: C.Winstanley{at}liverpool.ac.uk.

Journal of Clinical Microbiology, February 2000, p. 883-885, Vol. 38, No. 2
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

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