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Journal of Clinical Microbiology, February 2000, p. 914-917, Vol. 38, No. 2
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Comparative Evaluation of Two Branched-DNA Human Immunodeficiency Virus Type 1 RNA Quantification Assays with Lower Detection Limits of 50 and 500 Copies per Milliliter

Christoph Manegold,1,* Catharina Krempe,2 Helmut Jablonowski,1 Lasse Kajala,1 Manfred Dietrich,dagger and Ortwin Adams2

Klinik für Gastroenterologie, Hepatologie und Infektiologie1 and Institut für Medizinische Mikrobiologie und Virologie,2 Heinrich-Heine-Universität, Düsseldorf, Germany

Received 5 March 1999/Returned for modification 25 March 1999/Accepted 1 November 1999

We have comparatively evaluated Quantiplex version 3.0 and version 2.0 on 133 plasma samples and a repetitive dilution series. Version 3.0 yielded higher human immunodeficiency virus RNA values, and the ratio of version 3.0 results to version 2.0 results decreased from 3.47 below 1,000 copies/ml to 1.97 above 50,000 copies/ml [linear regression, log (version 3.0) = 0.915 + 0.871 × log (version 2.0); r2 = 0.952].

* Corresponding author. Present address: Bernhard-Nocht-Institut für Tropenmedizin, Bernhard-Nocht-Strasse 74, 20359 Hamburg, Germany. Phone: 49/40/42818-0 or -370. Fax: 49/40/42818-379. E-mail: manegold{at}bni.uni-hamburg.de.

dagger Present address: Bernhard-Nocht-Institut für Tropenmedizin, 20359 Hamburg, Germany.

Journal of Clinical Microbiology, February 2000, p. 914-917, Vol. 38, No. 2
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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