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Journal of Clinical Microbiology, February 2000, p. 923-925, Vol. 38, No. 2
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

PCR Using 3'-Mismatched Primers To Detect A2142C Mutation in 23S rRNA Conferring Resistance to Clarithromycin in Helicobacter pylori Clinical Isolates

Teresa Alarcón,* Diego Domingo, Nuria Prieto, and Manuel López-Brea

Department of Microbiology, Hospital Universitario de la Princesa, Madrid, Spain

Received 8 April 1999/Returned for modification 23 August 1999/Accepted 3 November 1999

Twenty-five clarithromycin-resistant Helicobacter pylori strains (selected by agar dilution) were studied to detect A2142G and A2143G mutations in the 23S rRNA gene by a PCR-restriction fragment length polymorphism method and an A2142C mutation by PCR using a 3'-mismatched specific primer. A 700-bp amplified fragment was obtained by the mismatched PCR only in strains without an A2142G or A2143G mutation, indicating that those strains had the A2142C mutation.

* Corresponding author. Mailing address: Department of Microbiology, Hospital Universitario de la Princesa, Diego de León 62, Madrid 28006, Spain. Phone: 34 91 309 00 47. Fax: 34 91 309 00 47. E-mail: microbiologia{at}hup.es.

Journal of Clinical Microbiology, February 2000, p. 923-925, Vol. 38, No. 2
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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